PARP1 Antibody 71 Publications

Rabbit Polyclonal| Catalog number: 13371-1-AP

Featured Product KD/KO validated

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Freight/Packing

Con: 28 μg/150 μl

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Species specificity:
human, mouse, rat, monkey, pig, sheep

Positive WB detected in:
Jurkat cells, K-562 cells, Raji cells, A549 cells, HeLa cells, Y79 cells, Fas antibody treated HeLa cells, Cobalt Chloride treated HeLa cells, C6 cells

Positive IP detected in:
K-562 cells

Positive IHC detected in:
human liver cancer tissue, human breast cancer tissue

Recommended dilution:
WB : 1:300-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:500

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
poly (ADP-ribose) polymerase 1

Calculated molecular weight:
1014aa,113 kDa

Observed molecular weight:
113-116, 89 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
PARP1

Synonyms
ADPRT, ADPRT 1, ADPRT1, pADPRT 1, PARP, PARP 1, PARP1, poly (ADP ribose) polymerase 1, Poly [ADP ribose] polymerase 1, Poly[ADP ribose] synthase 1, PPOL
Background

PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered as an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and gave rise to fragments ranging from 42-89-kD. This antibody was generated against the C-terminal region of human PARP1 and it recognizes the full-length as well as the cleavage of the PARP1.


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