PARP1 Polyclonal antibody

PARP1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13371-1-AP

Synonyms

ADPRT, ADPRT 1, ADPRT1, pADPRT 1, PARP, PARP 1, PARP1, poly (ADP ribose) polymerase 1, Poly [ADP ribose] polymerase 1, Poly[ADP ribose] synthase 1, PPOL



Tested Applications

Positive WB detected inJurkat cells, Cobalt Chloride treated HeLa cells, C6 cells, Fas antibody treated HeLa cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNeuro-2a cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13371-1-AP targets PARP1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, M. grisea, monkey, mouse, pig, rat, sheep, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PARP1 fusion protein Ag4193
Full Name poly (ADP-ribose) polymerase 1
Calculated molecular weight 1014 aa, 113 kDa
Observed molecular weight 113-116 kDa, 89 kDa
GenBank accession numberBC037545
Gene symbol PARP1
Gene ID (NCBI) 142
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered as an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and gave rise to fragments ranging from 42-89-kDa. This antibody was generated against the C-terminal region of human PARP1 and it recognizes the full-length as well as the cleavage of the PARP1.

Protocols

Product Specific Protocols
WB protocol for PARP1 antibody 13371-1-APDownload protocol
IHC protocol for PARP1 antibody 13371-1-APDownload protocol
IF protocol for PARP1 antibody 13371-1-APDownload protocol
IP protocol for PARP1 antibody 13371-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cancers (Basel)

Antrocin Sensitizes Prostate Cancer Cells to Radiotherapy through Inhibiting PI3K/AKT and MAPK Signaling Pathways.

Authors - Yu-An Chen
humanWB

Med Sci Monit

PMID: 30605443

Authors - Lin Chen
humanWB

J Cell Physiol

Rg3 inhibits gemcitabine-induced lung cancer cell invasiveness through ROS-dependent, NF-κB- and HIF-1α-mediated downregulation of PTX3.

Authors - Bulbul Ahmmed
humanWB

J Cell Mol Med

Tumour necrosis factor-α-induced protein 8-like 2 is a novel regulator of proliferation, migration, and invasion in human rectal adenocarcinoma cells.

Authors - Dong-Dong Wu
humanWB

Toxins (Basel)

Autophagy and Apoptosis Interact to Modulate T-2 Toxin-Induced Toxicity in Liver Cells.

Authors - Jing Wu
humanWB

Br J Pharmacol

Potent effects of dioscin against hepatocellular carcinoma through regulating TIGAR-mediated apoptosis, autophagy and DNA damage.

Authors - Zhang Mao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Brandon (Verified Customer) (02-01-2019)

Detected Full-length and cleaved PARP-1. Also detected a smaller fragment in mouse mouse liver tissue.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500 in 5% skim milk
  • Cell Tissue Type: C57BL/6 Mouse liver