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Pyruvate Carboxylase Antibody

Pyruvate Carboxylase Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16588-1-AP

Synonyms

PC, PCB, pyruvate carboxykinase, pyruvate Carboxylase, Pyruvic carboxylase



Tested Applications

Positive WB detected inmouse liver tissue, HepG2 cells, rat liver tissue
Positive IP detected inHepG2 cells, mouse liver tissue
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 8 publications below

Product Information

16588-1-AP targets Pyruvate Carboxylase in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Pyruvate Carboxylase fusion protein Ag8857
Full Name pyruvate carboxylase
Calculated molecular weight 1178aa,130 kDa
Observed molecular weight 125-130 kDa
GenBank accession number BC011617
Gene symbol PC
Gene ID (NCBI) 5091
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PC(pyruvate carboxylase) is a member of the family of biotin-dependent carboxylases and is found widely among eukaryotic tissues and in many prokaryotic species. It catalyses the ATP-dependent carboxylation of pyruvate to form oxaloacetate which may be utilised in the synthesis of glucose, fat, some amino acids or their derivatives and several neurotransmitters. Diabetes and hyperthyroidism increase the level of expression of pyruvate carboxylase in the long term, while its activity in the short term is controlled by the intramitochondrial concentrations of acetyl-CoA and pyruvate(PMID:9597748).

Protocols

Product Specific Protocols
WB protocol for Pyruvate Carboxylase antibody 16588-1-APDownload protocol
IHC protocol for Pyruvate Carboxylase antibody 16588-1-APDownload protocol
IF protocol for Pyruvate Carboxylase antibody 16588-1-APDownload protocol
IP protocol for Pyruvate Carboxylase antibody 16588-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Proteome Res

Integrated metabolomics and lipidomics analyses reveal metabolic reprogramming in human glioma with IDH1 mutation.

Authors - Lina Zhou
ratWB

Biol Pharm Bull

Proteomic analysis reveals the renoprotective effect of Tribulus terrestris against obesity-related glomerulopathy in rats.

Authors - Yue-Hua Jiang

Mol Biosyst

Correlated S-palmitoylation profiling of Snail-induced epithelial to mesenchymal transition.

Authors - Jeannie L Hernandez
pigWB

PLoS One

Betaine supplementation in maternal diet modulates the epigenetic regulation of hepatic gluconeogenic genes in neonatal piglets.

Authors - Demin Cai
humanWB

PLoS One

Mesenchymal phenotype predisposes lung cancer cells to impaired proliferation and redox stress in response to glutaminase inhibition.

Authors - Danielle B Ulanet
ratWB

J Nutr Biochem

High salt diet induces metabolic alterations in multiple biological processes of Dahl salt-sensitive rats.

Authors - Yanjun Wang