PD-1/CD279 Antibody

PD-1/CD279 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18106-1-AP

Synonyms

CD279, hPD 1, hPD l, PD 1, PD1, PD-1, PDCD1, programmed cell death 1, Protein PD 1, SLEB2



Tested Applications

Positive WB detected inmouse thymus tissue, mouse spleen tissue
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The 18106-1-AP immunogen is Fusion Protein PD-1/CD279 protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PD-1/CD279 fusion protein Ag12470
Full Name programmed cell death 1
Calculated molecular weight 288aa,32 kDa
Observed molecular weight 47-55 kDa
GenBank accession number BC074740
Gene symbol PDCD1
Gene ID (NCBI) 5133
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). It has been reported that PD-1 is heavily glycosylated and migrates with an apparent molecular mass of 47-55 kDa on SDS-PAGE , which is larger than its predicted mass of 32 kDa (PMID: 8671665; 17640856; 17003438).

Protocols

Product Specific Protocols
WB protocol for PD-1/CD279 antibody 18106-1-APDownload protocol
IHC protocol for PD-1/CD279 antibody 18106-1-APDownload protocol
IF protocol for PD-1/CD279 antibody 18106-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Oncol

PD-1/PD-L1 Axis, Rather Than High-Mobility Group Alarmins or CD8+ Tumor-Infiltrating Lymphocytes, Is Associated With Survival in Head and Neck Squamous Cell Carcinoma Patients Who Received Surgical Resection.

Authors - Fan Yang
mouseIF

NPJ Precis Oncol

PMID: 31482136

Authors - Natasha K Brockwell
humanIHC

Immunol Res

Immunological markers predict the prognosis of patients with squamous non-small cell lung cancer.

Authors - Long Jiang
humanIHC

Oncotarget

Co-expression of AFAP1-AS1 and PD-1 predicts poor prognosis in nasopharyngeal carcinoma.

Authors - Yanyan Tang
humanIHC

Int J Biol Markers

Expression of PD-1/PD-L1 in head and neck squamous cell carcinoma and its clinical significance.

Authors - Shu-Wei Chen
humanWB

Arch Gynecol Obstet

Programmed cell death protein 1 promotes hepatitis B virus transmission through the regulation of ERK1/2-mediated trophoblasts differentiation.

Authors - Jielian Yang