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PDE1C Polyclonal antibody

PDE1C Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 13785-1-AP

Synonyms

Cam PDE 1C, hCam 3, Hcam3, PDE1C

Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using PDE1C Polyclonal antibody (13785-1-AP)

    WB analysis of human heart using 13785-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 13785-1-AP (PDE1C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using PDE1C Polyclonal antibody (13785-1-AP)

    IP experiment of mouse heart using 13785-1-AP

    IP result of anti-PDE1C (IP:13785-1-AP, 4ug; Detection:13785-1-AP 1:500) with mouse heart tissue lysate 4000ug.

Tested Applications

Positive WB detected inhuman heart tissue
Positive IP detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

13785-1-AP targets PDE1C in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDE1C fusion protein Ag4798
Full Name phosphodiesterase 1C, calmodulin-dependent 70kDa
Calculated Molecular Weight 634 aa, 72 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC022525
Gene Symbol PDE1C
Gene ID (NCBI) 5137
RRIDAB_2268145
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclic nucleotide phosphodiesterases (PDEs) catalyze hydrolysis of the cyclic nucleotides cAMP and cGMP to the corresponding nucleoside 5-prime-monophosphates. PDE1C, is calmodulin-dependent PDEs (CaM-PDEs) that are stimulated by a calcium-calmodulin complex. Expression of PDE1C may be an important component in signal transduction pathways mediated by calcium that permit cell cycle traverse and proliferation(PMID:9366577). It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for PDE1C antibody 13785-1-APDownload protocol
IP protocol for PDE1C antibody 13785-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Sci Adv

RasGRP1 is a causal factor in the development of l-DOPA-induced dyskinesia in Parkinson's disease.

Authors - Mehdi Eshraghi
View Article
ratWB

Free Radic Biol Med

Poly (I:C) impairs NO donor-induced relaxation by overexposure to NO via the NF-kappa B/iNOS pathway in rat superior mesenteric arteries.

Authors - Makoto Ando
View Article