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PGK2 Polyclonal antibody

PGK2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13686-1-AP

Synonyms

PGK 2, PGK2, PGKB, PGKPS, phosphoglycerate kinase 2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using PGK2 Polyclonal antibody (13686-1-AP)

    WB analysis of mouse testis using 13686-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13686-1-AP (PGK2 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PGK2 Polyclonal antibody (13686-1-AP)

    WB analysis of HepG2 using 13686-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13686-1-AP (PGK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using PGK2 Polyclonal antibody (13686-1-AP)

    WB analysis of human testis using 13686-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 13686-1-AP (PGK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using PGK2 Polyclonal antibody (13686-1-AP)

    WB analysis of human heart using 13686-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 13686-1-AP (PGK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PGK2 Polyclonal antibody (13686-1-AP)

    WB analysis of HeLa using 13686-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13686-1-AP (PGK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using PGK2 Polyclonal antibody (13686-1-AP)

    WB analysis of mouse brain using 13686-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13686-1-AP (PGK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using PGK2 Polyclonal antibody (13686-1-AP)

    IHC staining of human testis using 13686-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13686-1-AP (PGK2 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PGK2 Polyclonal antibody (13686-1-AP)

    IHC staining of human testis using 13686-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13686-1-AP (PGK2 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using PGK2 Polyclonal antibody (13686-1-AP)

    IF Staining of mouse testis using 13686-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PGK2 antibody (13686-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using PGK2 Polyclonal antibody (13686-1-AP)

    IF Staining of mouse testis using 13686-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PGK2 antibody (13686-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse testis tissue, HeLa cells, HepG2 cells, human heart tissue, human testis tissue, mouse brain tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13686-1-AP targets PGK2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PGK2 fusion protein Ag4553
Full Name phosphoglycerate kinase 2
Calculated Molecular Weight 417 aa, 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC038843
Gene Symbol PGK2
Gene ID (NCBI) 5232
RRIDAB_2161237
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PGK2, phosphoglycerate kinase 2, which belongs to the phosphoglycerate kinase superfamily. It catalyzes the first ATP-generating step in the central metabolic pathway of glycolysis, converting 1,3-bisphosphoglycerate and ADP to 3-phosphoglycerate and ATP.

Protocols

Product Specific Protocols
WB protocol for PGK2 antibody 13686-1-APDownload protocol
IHC protocol for PGK2 antibody 13686-1-APDownload protocol
IF protocol for PGK2 antibody 13686-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Chemosphere

Arsenic influences spermatogenesis by disorganizing the elongation of spermatids in adult male mice.

Authors - Yongli Han
View Article
mouseWB

Cell Physiol Biochem

Effect of Type I Diabetes on the Proteome of Mouse Oocytes.

Authors - Guangjian Jiang
View Article
sheepWB

Theriogenology

Characterization of GALNTL5 gene sequence and expression in ovine testes and sperm.

Authors - Xiaolei Yao
View Article
mouseWB

Nature

A male germ-cell-specific ribosome controls male fertility

Authors - Huiling Li
View Article
humanWB

Cell Res

Low glucose metabolite 3-phosphoglycerate switches PHGDH from serine synthesis to p53 activation to control cell fate

Authors - Yu-Qing Wu
  • KD Validated
View Article