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PHF6 Polyclonal antibody

PHF6 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 51062-1-AP

Synonyms

BORJ, KIAA1823, PHD finger protein 6, PHD like zinc finger protein, PHF6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PHF6 Polyclonal antibody (51062-1-AP)

    WB analysis using 51062-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 51062-1-AP (PHF6 antibody) at dilution of 1:64000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PHF6 Polyclonal antibody (51062-1-AP)

    IHC staining of human ovary tumor using 51062-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 51062-1-AP (PHF6 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PHF6 Polyclonal antibody (51062-1-AP)

    IHC staining of human ovary tumor using 51062-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 51062-1-AP (PHF6 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using PHF6 Polyclonal antibody (51062-1-AP)

    IHC staining of human cervical cancer using 51062-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 51062-1-AP (PHF6 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using PHF6 Polyclonal antibody (51062-1-AP)

    IHC staining of human cervical cancer using 51062-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 51062-1-AP (PHF6 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PHF6 Polyclonal antibody (51062-1-AP)

    IF Staining of HeLa using 51062-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 51062-1-AP (PHF6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, K-562 cells
Positive IHC detected inhuman ovary tumor tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

51062-1-AP targets PHF6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHF6 fusion protein Ag2102
Full Name PHD finger protein 6
Calculated Molecular Weight365aa,41 kDa; 312aa,35 kDa
Observed Molecular Weight 41 kDa
GenBank Accession NumberBC005994
Gene Symbol PHF6
Gene ID (NCBI) 84295
RRIDAB_2165369
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PHF6 protein is highly conserved in vertebrates. Structurally, PHF6 protein contains two plant homeodomain (PHD)-like zinc fingers, two nuclear localization sequences and a nucleolar localization sequence, suggesting that PHF6 may play a role in the regulation of transcription. PHD zinc fingers are structurally conserved modules that interact with chromatin or mediate protein-protein interactions. PHF6 associates with subunits of the PAF1 transcription elongation complex including PAF1, LEO1, CTR9 and CDC73.The interaction of PHF6 with the PAF1 complex plays a critical role in PHF6 function in neuronal migration in the cerebral cortex.

Protocols

Product Specific Protocols
WB protocol for PHF6 antibody 51062-1-APDownload protocol
IHC protocol for PHF6 antibody 51062-1-APDownload protocol
IF protocol for PHF6 antibody 51062-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Leuk Res

MiR-1306-5p promotes cell proliferation and inhibits cell apoptosis in acute myeloid leukemia by downregulating PHF6 expression.

Authors - Xiang Gao
View Article