PI3K p110(alpha) Antibody

PI3K p110(alpha) Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20583-1-AP

Synonyms

p110 alpha, PI3 kinase subunit alpha, PI3K, PI3K alpha, PI3K p110(alpha), PI3-kinase p110 subunit alpha, PIK3CA, PtdIns 3 kinase subunit alpha



Tested Applications

Positive WB detected inHepG2 cells, COLO 320 cells, HeLa cells, A431 cells, mouse liver tissue
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

20583-1-AP targets PI3K p110(alpha) in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name phosphoinositide-3-kinase, catalytic, alpha polypeptide
Calculated molecular weight 124 kDa
Observed molecular weight 120-130 kDa
GenBank accession number NM_006218
Gene symbol PIK3CA
Gene ID (NCBI) 5290
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PIK3CA belongs to the PI3/PI4-kinase family. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. Defects in PIK3CA are associated with colorectal cancer (CRC). Defects in PIK3CA are associated with breast cancer. Defects in PIK3CA are associated with ovarian cancer. Defects in PIK3CA may underlie hepatocellular carcinoma (HCC). Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB). The antibody is specific to PIK3CA.

Publications

SpeciesApplicationTitle
mouseWB

Biomed Pharmacother

RACK1 promotes the proliferation, migration and invasion capacity of mouse hepatocellular carcinoma cell line in vitro probably by PI3K/Rac1 signaling pathway.

Authors - Wu Jun J

Biomed Pharmacother

Mitochondrial DNA content reduction induces aerobic glycolysis and reversible resistance to drug-induced apoptosis in SW480 colorectal cancer cells.

Authors - Jing-Jing Mou
WB

Dev Comp Immunol

Characterization, functional and signaling elucidation of pigeon (Columba livia) interferon-α: Knockdown p53 negatively modulates antiviral response.

Authors - Siwen Li
humanIHC

J Cancer

Vasculogenic Mimicry in Prostate Cancer: The Roles of EphA2 and PI3K.

Authors - Hua Wang
humanWB

Oncotarget

Epithelial but not stromal expression of collagen alpha-1(III) is a diagnostic and prognostic indicator of colorectal carcinoma.

Authors - Xiao-Qing Wang
humanWB,IHC

Oncogene

KIF26B, a novel oncogene, promotes proliferation and metastasis by activating the VEGF pathway in gastric cancer.

Authors - H Zhang