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PLCB3 Monoclonal antibody

PLCB3 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B8B3

Cat no : 66668-1-Ig

Synonyms

FLJ37084, Phospholipase C beta 3, PLC beta 3, PLCB3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PLCB3 Monoclonal antibody (66668-1-Ig)

    WB analysis using 66668-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66668-1-Ig (PLCB3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • Western Blot (WB) analysis of various lysates using PLCB3 Monoclonal antibody (66668-1-Ig)

    WB analysis using 66668-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66668-1-Ig (PLCB3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Sp2/0 cells using PLCB3 Monoclonal antibody (66668-1-Ig)

    WB analysis of Sp2/0 using 66668-1-Ig

    Sp2/0 cells were subjected to SDS PAGE followed by western blot with 66668-1-Ig (PLCB3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PLCB3 Monoclonal antibody (66668-1-Ig)

    WB analysis using 66668-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66668-1-Ig (PLCB3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using PLCB3 Monoclonal antibody (66668-1-Ig)

    WB analysis of Jurkat using 66668-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66668-1-Ig (PLCB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using PLCB3 Monoclonal antibody (66668-1-Ig)

    WB analysis of HSC-T6 using 66668-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66668-1-Ig (PLCB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using PLCB3 Monoclonal antibody (66668-1-Ig)

    WB analysis of NIH/3T3 using 66668-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66668-1-Ig (PLCB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PLCB3 Monoclonal antibody (66668-1-Ig)

    IHC staining of human breast cancer using 66668-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66668-1-Ig (PLCB3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PLCB3 Monoclonal antibody (66668-1-Ig)

    IHC staining of human breast cancer using 66668-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66668-1-Ig (PLCB3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using PLCB3 Monoclonal antibody (66668-1-Ig)

    IHC staining of human colon using 66668-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66668-1-Ig (PLCB3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using PLCB3 Monoclonal antibody (66668-1-Ig)

    IHC staining of human colon using 66668-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66668-1-Ig (PLCB3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, HeLa cells, Sp2/0 cells, human peripheral blood platelets, HEK-293 cells, K-562, LNCaP cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

66668-1-Ig targets PLCB3 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityrat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PLCB3 fusion protein Ag15845
Full Name phospholipase C, beta 3 (phosphatidylinositol-specific)
Calculated Molecular Weight 1234 aa, 139 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC142681
Gene Symbol PLCB3
Gene ID (NCBI) 5331
RRIDAB_2882022
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLCB3 is a member of the phosphoinositide phospholipase C beta enzyme family that catalyze the production of the secondary messengers diacylglycerol and inositol 1,4,5-triphosphate from phosphatidylinositol in G-protein-linked receptor-mediated signal transduction. Six subfamilies of PLCs (B, G, D, E, Z and H) constitute part of ubiquitous signaling cascades that translate hormonal signals into intracellular events, leading to alternations in cell function. PLCB isoforms 1-4 are stimulated by G-protein activation (Gαq/11 and/or Gβγ). Independent of its enzymatic activity, PLCB3 inhibits the proliferation of hematopoietic stem cells (HSCs) and myeloid cells.

Protocols

Product Specific Protocols
WB protocol for PLCB3 antibody 66668-1-IgDownload protocol
IHC protocol for PLCB3 antibody 66668-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

J Cell Mol Med

Prenatal hypoxia inhibited propionate-evoked BK channels of mesenteric artery smooth muscle cells in offspring.

Authors - Wenna Zhang
View Article
ratWB

J Food Biochem

A novel di-peptide Met-Glu from collagen hydrolysates inhibits platelet aggregation and thrombus formation via regulation of Gq-mediated signaling.

Authors - Yijie Yang
View Article
ratWB

J Food Sci

Tripeptide Hyp-Asp-Gly from collagen peptides inhibited platelet activation via regulation of PI3K/Akt-MAPK/ERK1/2 signaling pathway

Authors - Hongdong Song
View Article