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PLUNC Polyclonal antibody

PLUNC Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB,IP,IHC,ELISA

Conjugate

Unconjugated

Cat no : 10413-1-AP

Synonyms

BPIFA1, LPLUNC3, Lung specific protein X, LUNX, NASG, PLUNC, Protein Plunc, SPLUNC1, SPURT, Von Ebner protein Hl

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using PLUNC Polyclonal antibody (10413-1-AP)

    WB analysis of mouse lung using 10413-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 10413-1-AP (PLUNC antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse lung tissue using PLUNC Polyclonal antibody (10413-1-AP)

    IP experiment of mouse lung using 10413-1-AP

    IP result of anti-PLUNC (IP:10413-1-AP, 4ug; Detection:10413-1-AP 1:300) with mouse lung tissue lysate 3600ug.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using PLUNC Polyclonal antibody (10413-1-AP)

    IHC staining of mouse lung using 10413-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10413-1-AP (PLUNC antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using PLUNC Polyclonal antibody (10413-1-AP)

    IHC staining of mouse lung using 10413-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10413-1-AP (PLUNC antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PLUNC Polyclonal antibody (10413-1-AP)

    IHC staining of human lung cancer using 10413-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10413-1-AP (PLUNC Antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PLUNC Polyclonal antibody (10413-1-AP)

    IHC staining of human lung cancer using 10413-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10413-1-AP (PLUNC Antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inmouse lung tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inmouse lung tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10413-1-AP targets PLUNC in WB,IP,IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLUNC fusion protein Ag0629
Full Name palate, lung and nasal epithelium associated
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC012549
Gene Symbol PLUNC
Gene ID (NCBI) 51297
RRIDAB_2166093
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLUNC, also named as LUNX, NASG and SPURT, belongs to the BPI/LBP/Plunc superfamily and Plunc family. PLUNC may be involved in the airway inflammatory response after exposure to irritants. It is associated with tumor progression. PLUNC plays a role in innate immune responses of the upper airways.

Protocols

Product Specific Protocols
WB protocol for PLUNC antibody 10413-1-APDownload protocol
IHC protocol for PLUNC antibody 10413-1-APDownload protocol
IP protocol for PLUNC antibody 10413-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Sci Rep

Single-cell transcriptomes reveal heterogeneity of chlorine-induced mice acute lung injury and the inhibitory effect of pentoxifylline on ferroptosis

Authors - Chen-Qian Zhao
View Article
mouse,humanWB,IHC,IF,ChIP

Cell Death Dis

USP7 inhibits the progression of nasopharyngeal carcinoma via promoting SPLUNC1-mediated M1 macrophage polarization through TRIM24

Authors - Huai Liu
View Article
human,mouseWB

Front Immunol

CC16 drives VLA-2-dependent SPLUNC1 expression

Authors - Natalie Iannuzo
View Article
humanWB

Med Oncol

SPLUNC1 regulates LPS-induced progression of nasopharyngeal carcinoma and proliferation of myeloid-derived suppressor cells

Authors - Ling Tang
View Article