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PLZF Monoclonal antibody

PLZF Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2E6F12

Cat no : 66672-1-Ig

Synonyms

PLZF, ZBTB16, Zinc finger protein 145, Zinc finger protein PLZF, ZNF145

Validation Data Gallery

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  • Western Blot (WB) analysis of Ramos cells using PLZF Monoclonal antibody (66672-1-Ig)

    WB analysis of Ramos using 66672-1-Ig

    Ramos cells were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using PLZF Monoclonal antibody (66672-1-Ig)

    WB analysis of human testis using 66672-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Ramos cells using PLZF Monoclonal antibody (66672-1-Ig)

    WB analysis of Ramos using 66672-1-Ig

    Ramos cells were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:7500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using PLZF Monoclonal antibody (66672-1-Ig)

    WB analysis of Raji using 66672-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:7500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Daudi cells using PLZF Monoclonal antibody (66672-1-Ig)

    WB analysis of Daudi using 66672-1-Ig

    Daudi cells were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:7500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PLZF Monoclonal antibody (66672-1-Ig)

    WB analysis of HeLa using 66672-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of THP-1 cells using PLZF Monoclonal antibody (66672-1-Ig)

    WB analysis of THP-1 using 66672-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 66672-1-Ig (PLZF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inRamos cells, Raji cells, Daudi cells, HeLa cells, THP-1 cells, human testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

66672-1-Ig targets PLZF in WB, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PLZF fusion protein Ag25180
Full Name zinc finger and BTB domain containing 16
Calculated Molecular Weight 673 aa, 74 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC026902
Gene Symbol ZBTB16
Gene ID (NCBI) 7704
RRIDAB_2882026
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLZF, also named as Zinc finger protein 145, is a 673 amino acid protein, which belongs to the krueppel C2H2-type zinc-finger protein family. PLZF is expressed in bone marrow, early myeloid cell lines and peripheral blood mononuclear cells. PLZF may play a role in myeloid maturation and in the development and/or maintenance of other differentiated tissues.

Protocols

Product Specific Protocols
WB protocol for PLZF antibody 66672-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Physiol

Abscopal effects of thoracic X-ray radiation on spermatogenesis in mice

Authors - Pan-Pan Lai
View Article
mouseWB

iScience

Chromodomain helicase DNA-binding domain 2 maintains spermatogonial self-renewal by promoting chromatin accessibility and mRNA stability

Authors - Ziqian Min
View Article
mouseWB

J Hazard Mater

Prenatal and postnatal exposure to polystyrene microplastics induces testis developmental disorder and affects male fertility in mice

Authors - Tianxin Zhao
View Article