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  • KD/KO Validated

PPAR gamma Antibody

PPAR gamma Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP, CHIP, ELISA

Conjugate

Unconjugated

Cat no : 16643-1-AP

Synonyms

CIMT1, NR1C3, PPAR gamma, PPARG, PPARG1, PPARG2, PPARgamma, PPARγ



Tested Applications

Positive WB detected inU-937 cells, HL-60 cells, human heart tissue, K-562 cells, MCF-7 cells, mouse heart tissue
Positive IP detected inHL-60 cells
Positive IHC detected inhuman prostate cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in3T3-L1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16643-1-AP targets PPAR gamma in WB, IP, IHC, IF, CoIP, CHIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity hamster, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPAR gamma fusion protein Ag10005
Full Name peroxisome proliferator-activated receptor gamma
Calculated molecular weight 58 kDa
Observed molecular weight 50-60 kDa
GenBank accession number BC006811
Gene symbol PPARG
Gene ID (NCBI) 5468
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Peroxisome Proliferator-Activated Receptors (PPARs) are ligand-activated intracellular transcription factors, members of the nuclear hormone receptor superfamily (NR), that includes estrogen, thyroid hormone receptors, retinoic acid, Vitamin D3 as well as retinoid X receptors (RXRs). The PPAR subfamily consists of three subtypes encoded by distinct genes denoted PPARα (NR1C1), PPARβ/δ (NR1C2) and PPARγ (NR1C3), which are activated by selective ligands. PPARγ, also named as PPARG, contains one nuclear receptor DNA-binding domain and is a receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. It plays an important role in the regulation of lipid homeostasis, adipogenesis, ins resistance, and development of various organs. Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) and may be associated with susceptibility to obesity. Defects in PPARG can lead to type 2 ins-resistant diabetes and hypertension. PPARG mutations may be associated with colon cancer. Genetic variations in PPARG are associated with susceptibility to glioma type 1 (GLM1). PPARG has two isoforms with molecular weight 57 kDa and 54 kDa (PMID: 9831621), but modified PPARG is about 67 KDa (PMID: 16809887). PPARG2 is a splice variant and has an additional 30 amino acids at the N-terminus (PMID: 15689403). Experimental data indicate that a 45 kDa protein displaying three different sequences immunologically related to the nuclear receptor PPARG2 is located in mitochondria (mt-PPAR). However, the molecular weight of this protein is clearly less when compared to that of PPARG2 (57 kDa) (PMID: 10922459). PPARG has been reported to be localized mainly (but not always) in the nucleus. PPARG can also be detected in the cytoplasm and was reported to possess extra-nuclear/non-genomic actions (PMID: 17611413; 19432669; 14681322).

Protocols

Product Specific Protocols
WB protocol for PPAR gamma antibody 16643-1-APDownload protocol
IHC protocol for PPAR gamma antibody 16643-1-APDownload protocol
IF protocol for PPAR gamma antibody 16643-1-APDownload protocol
IP protocol for PPAR gamma antibody 16643-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Stem Cell Res Ther

Catalpol promotes the osteogenic differentiation of bone marrow mesenchymal stem cells via the Wnt/β-catenin pathway.

Authors - Yu Zhu
ratWB

Biomed Pharmacother

Protective effect of cultured bear bile powder against dimethylnitrosamine-induced hepatic fibrosis in rats.

Authors - Min Zheng
mouseWB

Int J Med Sci

MicroRNA-29a is a key regulon that regulates BRD4 and mitigates liver fibrosis in mice by inhibiting hepatic stellate cell activation.

Authors - Ying-Hsien Huang
ratWB

Eur J Pharmacol

PMID: 30797787

Authors - Honglei Zhang
humanWB

Chem Res Toxicol

Organophosphorus Flame Retardants Impair Intracellular Lipid Metabolic Function in Human Hepatocellular Cells.

Authors - Zhengliang Hao
humanWB

Sci Rep

Evaluation of MEDAG gene expression in papillary thyroid microcarcinoma: associations with histological features, regional lymph node metastasis and prognosis.

Authors - Yang Song

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Boyan (Verified Customer) (04-24-2019)

By WB, this antibody labeled many bands, which makes it very hard to distinguish which one is the actual PPARg.

  • Applications: Western Blot,
  • Cell Tissue Type: 3T3 L1
No image submitted with review