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PPAR Gamma Polyclonal antibody
PPAR Gamma Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Cat no : 16643-1-AP
|Positive WB detected in||U-937 cells, HL-60 cells, human heart tissue, K-562 cells, MCF-7 cells, mouse heart tissue|
|Positive IP detected in||HL-60 cells|
|Positive IHC detected in||human prostate cancer tissue, mouse colon tissue, human placenta tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||3T3-L1 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
16643-1-AP targets PPAR Gamma in WB, IP, IHC, IF, FC, CoIP, CHIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||hamster, human, mouse, rat, sheep|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PPAR Gamma fusion protein Ag10005|
|Full Name||peroxisome proliferator-activated receptor gamma|
|Calculated molecular weight||58 kDa|
|Observed molecular weight||50-60 kDa|
|GenBank accession number||BC006811|
|Gene ID (NCBI)||5468|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Peroxisome Proliferator-Activated Receptors (PPARs) are ligand-activated intracellular transcription factors, members of the nuclear hormone receptor superfamily (NR), that includes estrogen, thyroid hormone receptors, retinoic acid, Vitamin D3 as well as retinoid X receptors (RXRs). The PPAR subfamily consists of three subtypes encoded by distinct genes denoted PPARα (NR1C1), PPARβ/δ (NR1C2) and PPARγ (NR1C3), which are activated by selective ligands. PPARγ, also named as PPARG, contains one nuclear receptor DNA-binding domain and is a receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. It plays an important role in the regulation of lipid homeostasis, adipogenesis, ins resistance, and development of various organs. Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) and may be associated with susceptibility to obesity. Defects in PPARG can lead to type 2 ins-resistant diabetes and hypertension. PPARG mutations may be associated with colon cancer. Genetic variations in PPARG are associated with susceptibility to glioma type 1 (GLM1). PPARG has two isoforms with molecular weight 57 kDa and 54 kDa (PMID: 9831621), but modified PPARG is about 67 KDa (PMID: 16809887). PPARG2 is a splice variant and has an additional 30 amino acids at the N-terminus (PMID: 15689403). Experimental data indicate that a 45 kDa protein displaying three different sequences immunologically related to the nuclear receptor PPARG2 is located in mitochondria (mt-PPAR). However, the molecular weight of this protein is clearly less when compared to that of PPARG2 (57 kDa) (PMID: 10922459). PPARG has been reported to be localized mainly (but not always) in the nucleus. PPARG can also be detected in the cytoplasm and was reported to possess extra-nuclear/non-genomic actions (PMID: 17611413; 19432669; 14681322).
|Product Specific Protocols|
|WB protocol for PPAR Gamma antibody 16643-1-AP||Download protocol|
|IHC protocol for PPAR Gamma antibody 16643-1-AP||Download protocol|
|IF protocol for PPAR Gamma antibody 16643-1-AP||Download protocol|
|IP protocol for PPAR Gamma antibody 16643-1-AP||Download protocol|
|FC protocol for PPAR Gamma antibody 16643-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Boyan (Verified Customer) (04-24-2019)
By WB, this antibody labeled many bands, which makes it very hard to distinguish which one is the actual PPARg.