PPAR gamma Antibody 35 Publications

Rabbit Polyclonal| Catalog number: 16643-1-AP

Featured Product KD/KO validated

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Freight/Packing

Con: 49 μg/150 μl

Choose size:

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Species specificity:
human, mouse, rat, fish

Positive WB detected in:
U-937 cells, HL-60 cells, human heart tissue, K-562 cells, MCF-7 cells, mouse heart tissue

Positive IP detected in:
HL-60 cells

Positive IHC detected in:
human prostate cancer tissue, human heart tissue

Positive IF detected in:
HepG2 cells

Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:200
IF : 1:10-1:100

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
peroxisome proliferator-activated receptor gamma

Calculated molecular weight:
58 kDa

Observed molecular weight:
50-60 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
PPARG

Synonyms
CIMT1, NR1C3, PPAR gamma, PPARG, PPARG1, PPARG2, PPARgamma
Background

eroxisome Proliferator-Activated Receptors (PPARs) are ligand-activated intracellular transcription factors, members of the nuclear hormone receptor superfamily (NR), that include estrogen, thyroid hormone receptors, retinoic acid, Vitamin D3 as well as retinoid X receptors (RXRs). The PPAR subfamily consists of three subtypes encoded by distinct genes denoted PPARα (NR1C1), PPARβ/δ (NR1C2) and PPARγ (NR1C3), which are activated by selective ligands. PPARγ, also named as PPARG, contains one nuclear receptor DNA-binding domain and is a receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. It plays an important role in the regulation of lipid homeostasis, adipogenesis, insulin resistance, and development of various organs. Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) and may be associated with susceptibility to obesity (OBESITY). Defects in PPARG can lead to type 2 insulin-resistant diabetes and hyptertension. PPARG mutations may be associated with colon cancer. Genetic variations in PPARG can be associated with susceptibility to glioma type 1 (GLM1). PPARG exists two isoforms with MV 57 kDa and 54 kDa (PMID: 9831621), but modified PPARG is about 67 KDa (PMID: 168098870). PPARG2 is a splice variant and has an additional 30 amino acids at the N-terminus. (PMID: 15689403 ). The experiment indicates that a 45 kDa protein displaying three different sequences immunologically related to the nuclear receptor PPARG2 is located in mitochondria (mt-PPAR). However, the molecular weight of this protein is clearly less when compared to that of PPARG2 (57 kDa). (PMID: 10922459).


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