PPP3CA Polyclonal antibody

PPP3CA Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 13422-1-AP

Synonyms

Calcineurin A alpha, CALN, CALNA, CALNA1, CAM PRP catalytic subunit, CCN1, CNA, CNA1, PPP2B, PPP3CA



Tested Applications

Positive WB detected inmouse skeletal muscle tissue, mouse heart tissue, SH-SY5Y cells
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman colon cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

13422-1-AP targets PPP3CA in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity chicken, human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP3CA fusion protein Ag4203
Full Name protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform
Calculated molecular weight 59 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC025714
Gene symbol PPP3CA
Gene ID (NCBI) 5530
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PPP3CA, also called calcineurin Aα, is the only serine/threonine protein phosphatase under the control of Ca2+/calmodulin, and plays a critical role in the coupling of Ca2+ signals to cellular responses. Ca2+ signaling plays a central role in hypertrophic growth of cardiac and skeletal muscle in response to mechanical load and a variety of signals. Therefore, PPP3CA plays an important role in muscle differentiation, especially in muscle fiber type conversion. It is also involved in osteoclast regulation, regulating bone formation through an effect on osteoblast differentiation.

Protocols

Product Specific Protocols
WB protocol for PPP3CA antibody 13422-1-APDownload protocol
IHC protocol for PPP3CA antibody 13422-1-APDownload protocol
IF protocol for PPP3CA antibody 13422-1-APDownload protocol
IP protocol for PPP3CA antibody 13422-1-APDownload protocol
FC protocol for PPP3CA antibody 13422-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Acta Histochem

Effect of Hypoxia on the Muscle Fiber Switching Signal Pathways CnA/NFATc1 and Myostatin in Mouse Myocytes.

Authors - Li Lixin
mouseWB

JCI Insight

MicroRNA-30 regulates left ventricular hypertrophy in chronic kidney disease.

Authors - Jingfu Bao
chickenWB

Biol Trace Elem Res

Transcriptional Study Revealed That Boron Supplementation May Alter the Immune-Related Genes Through MAPK Signaling in Ostrich Chick Thymus.

Authors - Ke Xiao
humanWB,IF

Neurochem Res

Inhibition of Calcineurin A by FK506 Suppresses Seizures and Reduces the Expression of GluN2B in Membrane Fraction.

Authors - Yuetao Wen
mouseWB

Eur J Pharmacol

Stachydrine hydrochloride suppresses phenylephrine-induced pathological cardiac hypertrophy by inhibiting the calcineurin/nuclear factor of activated T-cell signalling pathway.

Authors - Jiali Zheng
humanWB,IF

Neurobiol Dis

Calcineurin β protects brain after injury by activating the unfolded protein response.

Authors - Yanan Chen