Validation Data Gallery
|Positive WB detected in||mouse skeletal muscle tissue, mouse heart tissue, SH-SY5Y cells|
|Positive IP detected in||mouse heart tissue|
|Positive IHC detected in||human colon cancer tissue, human cervical cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||SH-SY5Y cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
13422-1-AP targets PPP3CA in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||chicken, human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PPP3CA fusion protein Ag4203|
|Full Name||protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform|
|Calculated molecular weight||59 kDa|
|Observed molecular weight||59 kDa|
|GenBank accession number||BC025714|
|Gene ID (NCBI)||5530|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PPP3CA, also called calcineurin Aα, is the only serine/threonine protein phosphatase under the control of Ca2+/calmodulin, and plays a critical role in the coupling of Ca2+ signals to cellular responses. Ca2+ signaling plays a central role in hypertrophic growth of cardiac and skeletal muscle in response to mechanical load and a variety of signals. Therefore, PPP3CA plays an important role in muscle differentiation, especially in muscle fiber type conversion. It is also involved in osteoclast regulation, regulating bone formation through an effect on osteoblast differentiation.
|Product Specific Protocols|
|WB protocol for PPP3CA antibody 13422-1-AP||Download protocol|
|IHC protocol for PPP3CA antibody 13422-1-AP||Download protocol|
|IF protocol for PPP3CA antibody 13422-1-AP||Download protocol|
|IP protocol for PPP3CA antibody 13422-1-AP||Download protocol|
|FC protocol for PPP3CA antibody 13422-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Effect of Hypoxia on the Muscle Fiber Switching Signal Pathways CnA/NFATc1 and Myostatin in Mouse Myocytes.
MicroRNA-30 regulates left ventricular hypertrophy in chronic kidney disease.
Biol Trace Elem Res
Transcriptional Study Revealed That Boron Supplementation May Alter the Immune-Related Genes Through MAPK Signaling in Ostrich Chick Thymus.
Inhibition of Calcineurin A by FK506 Suppresses Seizures and Reduces the Expression of GluN2B in Membrane Fraction.
Eur J Pharmacol
Stachydrine hydrochloride suppresses phenylephrine-induced pathological cardiac hypertrophy by inhibiting the calcineurin/nuclear factor of activated T-cell signalling pathway.
Calcineurin β protects brain after injury by activating the unfolded protein response.