PPP3CA Polyclonal antibody

PPP3CA Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 13422-1-AP

Synonyms

Calcineurin A alpha, CALN, CALNA, CALNA1, CAM PRP catalytic subunit, CCN1, CNA, CNA1, PP2BA, PPP2B, PPP3CA



Tested Applications

Positive WB detected inmouse skeletal muscle tissue, RAW 264.7 cells, mouse heart tissue, SH-SY5Y cells
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman colon cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13422-1-AP targets PPP3CA in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, chicken, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP3CA fusion protein Ag4203
Full Name protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform
Calculated Molecular Weight 59 kDa
Observed Molecular Weight 59 kDa
GenBank Accession NumberBC025714
Gene Symbol PPP3CA
Gene ID (NCBI) 5530
RRIDAB_2168314
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPP3CA, also called calcineurin Aα, is the only serine/threonine protein phosphatase under the control of Ca2+/calmodulin, and plays a critical role in the coupling of Ca2+ signals to cellular responses. Ca2+ signaling plays a central role in hypertrophic growth of cardiac and skeletal muscle in response to mechanical load and a variety of signals. Therefore, PPP3CA plays an important role in muscle differentiation, especially in muscle fiber type conversion. It is also involved in osteoclast regulation, regulating bone formation through an effect on osteoblast differentiation.

Protocols

Product Specific Protocols
WB protocol for PPP3CA antibody 13422-1-APDownload protocol
IHC protocol for PPP3CA antibody 13422-1-APDownload protocol
IF protocol for PPP3CA antibody 13422-1-APDownload protocol
IP protocol for PPP3CA antibody 13422-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Psychiatry

A recurrent SHANK1 mutation implicated in autism spectrum disorder causes autistic-like core behaviors in mice via downregulation of mGluR1-IP3R1-calcium signaling.

Authors - Yue Qin
mouseWB

Proc Natl Acad Sci U S A

Major contribution of the 3/6/7 class of TRPC channels to myocardial ischemia/reperfusion and cellular hypoxia/reoxygenation injuries.

Authors - Xiju He
pigWB

J Agric Food Chem

Dietary Taurine Supplementation Improves the Meat Quality, Muscle Fiber Type, and Mitochondrial Function of Finishing Pigs

Authors - Wenting An
pigWB

J Cell Physiol

Taurine promotes muscle fiber type transformation through CaN/NFATc1 signaling in porcine myoblasts

Authors - Wenting An
humanWB,IF

Neurobiol Dis

Calcineurin β protects brain after injury by activating the unfolded protein response.

Authors - Yanan Chen
mouseWB

Eur J Pharmacol

Stachydrine hydrochloride suppresses phenylephrine-induced pathological cardiac hypertrophy by inhibiting the calcineurin/nuclear factor of activated T-cell signalling pathway.

Authors - Jiali Zheng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

nuo (Verified Customer) (08-22-2021)

A very good ab to probe PPP3CA by WB. With a high titer.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: mouse brain lysate
PPP3CA Antibody Western Blot validation (1:3000 dilution) in mouse brain lysate (Cat no:13422-1-AP)