AMPK Gamma 2 Polyclonal antibody

AMPK Gamma 2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12568-1-AP

Synonyms

AAKG, AAKG2, AMPK gamma2, AMPK subunit gamma 2, CMH6, H91620p, PRKAG2, WPWS



Tested Applications

Positive WB detected inmouse heart tissue, HEK-293 cells, HeLa cells, mouse eye tissue, PC-3 cells
Positive IHC detected inhuman heart tissue, human ovary tumor tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

12568-1-AP targets AMPK Gamma 2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AMPK Gamma 2 fusion protein Ag3214
Full Name protein kinase, AMP-activated, gamma 2 non-catalytic subunit
Calculated molecular weight 569 aa, 63 kDa
Observed molecular weight 63 kDa
GenBank accession numberBC020540
Gene symbol PRKAG2
Gene ID (NCBI) 51422
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit, a noncatalytic beta subunit, and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist, encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. AMPK gamma2 is a member of the AMPK gamma subunit family. Mutations in this gene have been associated with Wolff-Parkinson-White syndrome, familial hypertrophic cardiomyopathy, and glycogen storage disease of the heart.

Protocols

Product Specific Protocols
WB protocol for AMPK Gamma 2 antibody 12568-1-APDownload protocol
IHC protocol for AMPK Gamma 2 antibody 12568-1-APDownload protocol
IF protocol for AMPK Gamma 2 antibody 12568-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

J Cardiol

Identification and functional analysis of a novel PRKAG2 mutation responsible for Chinese PRKAG2 cardiac syndrome reveal an important role of non-CBS domains in regulating the AMPK pathway.

Authors - Zhang Bi-Li BL