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  • Featured Product
  • KD/KO Validated

PRMT5 Polyclonal antibody

PRMT5 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 18436-1-AP

Synonyms

72 kDa ICln binding protein, HRMT1L5, IBP72, Jak binding protein 1, JBP1, PRMT5, SKB1, SKB1 homolog, SKB1Hs

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRMT5 Polyclonal antibody (18436-1-AP)

    WB analysis using 18436-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18436-1-AP (PRMT5 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PRMT5 Polyclonal antibody (18436-1-AP)

    WB analysis using 18436-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18436-1-AP (PRMT5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PRMT5 Polyclonal antibody (18436-1-AP)

    WB analysis using 18436-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18436-1-AP (PRMT5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse embryo tissue using PRMT5 Polyclonal antibody (18436-1-AP)

    WB analysis of mouse embryo using 18436-1-AP

    mouse embryo tissue were subjected to SDS PAGE followed by western blot with 18436-1-AP (PRMT5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using PRMT5 Polyclonal antibody (18436-1-AP)

    WB analysis of Raji using 18436-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 18436-1-AP (PRMT5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using PRMT5 Polyclonal antibody (18436-1-AP)

    WB analysis of Jurkat using 18436-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 18436-1-AP (PRMT5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using PRMT5 Polyclonal antibody (18436-1-AP)

    IP experiment of mouse brain using 18436-1-AP

    IP result of anti-PRMT5 (IP:18436-1-AP, 4ug; Detection:18436-1-AP 1:2000) with mouse brain tissue lysate 1280 ug.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PRMT5 Polyclonal antibody (18436-1-AP)

    IHC staining of mouse liver using 18436-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PRMT5 Polyclonal antibody (18436-1-AP)

    IHC staining of human breast cancer using 18436-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PRMT5 Polyclonal antibody (18436-1-AP)

    IHC staining of human breast cancer using 18436-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using PRMT5 Polyclonal antibody (18436-1-AP)

    IHC staining of human lung using 18436-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using PRMT5 Polyclonal antibody (18436-1-AP)

    IHC staining of human lung using 18436-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PRMT5 Polyclonal antibody (18436-1-AP)

    IHC staining of human colon cancer using 18436-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PRMT5 Polyclonal antibody (18436-1-AP)

    IHC staining of human colon cancer using 18436-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using PRMT5 Polyclonal antibody (18436-1-AP)

    IHC staining of human lymphoma using 18436-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using PRMT5 Polyclonal antibody (18436-1-AP)

    IHC staining of human lymphoma using 18436-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PRMT5 Polyclonal antibody (18436-1-AP)

    IHC staining of human lung cancer using 18436-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PRMT5 Polyclonal antibody (18436-1-AP)

    IHC staining of human lung cancer using 18436-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PRMT5 Polyclonal antibody (18436-1-AP)

    IF Staining of HeLa using 18436-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRMT5 antibody (18436-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PRMT5 Polyclonal antibody (18436-1-AP)

    IF Staining of HepG2 using 18436-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PRMT5 antibody (18436-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, mouse embryo tissue, Raji cells, HepG2 cells, Jurkat cells, NIH/3T3 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human lymphoma tissue, human lung tissue, human colon cancer tissue, human lung cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18436-1-AP targets PRMT5 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRMT5 fusion protein Ag9736
Full Name protein arginine methyltransferase 5
Calculated Molecular Weight 637 aa, 73 kDa
Observed Molecular Weight 72 kDa
GenBank Accession NumberBC025979
Gene Symbol PRMT5
Gene ID (NCBI) 10419
RRIDAB_2171798
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRMT5 is a Type II enzyme that catalyzes arginine monomethylation and symmetric dimethylation (Rme1 and Rme2s). The many targets of PRMT5 include ribosomal proteins, the histone chaperone nucleoplasmin, p53, and histones. PRMT5 is frequently observed in a complex with the cofactor, methylosome protein 50 (MEP50), which is required for PRMT5 activity. PRMT5 is upregulated in several human malignancies, including lymphomas, lung cancer, breast cancer and colorectal cancer.

Protocols

Product Specific Protocols
WB protocol for PRMT5 antibody 18436-1-APDownload protocol
IHC protocol for PRMT5 antibody 18436-1-APDownload protocol
IF protocol for PRMT5 antibody 18436-1-APDownload protocol
IP protocol for PRMT5 antibody 18436-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Adv

Arginine methyltransferase PRMT5 negatively regulates cGAS-mediated antiviral immune response.

Authors - Dapeng Ma
  • KD Validated
View Article
humanWB

Nat Commun

The LINC01138 drives malignancies via activating arginine methyltransferase 5 in hepatocellular carcinoma.

Authors - Zhe Li
View Article
humanWB,IP

Nat Commun

USP9X regulates centrosome duplication and promotes breast carcinogenesis.

Authors - Xin Li
View Article
humanWB,IP

Cell Death Dis

MYBL1 induces transcriptional activation of ANGPT2 to promote tumor angiogenesis and confer sorafenib resistance in human hepatocellular carcinoma

Authors - Jinrong Zhu
View Article
humanWB,IHC

Aging (Albany NY)

High PRMT5 expression is associated with poor overall survival and tumor progression in bladder cancer.

Authors - Lei Tan
  • KD Validated
View Article
humanWB

Exp Ther Med

Protein arginine methyltransferase 5 mediates THP-1-derived macrophage activation dependent on NF-κB in endometriosis.

Authors - Xiaoshan Chai
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Satyendra (Verified Customer) (07-16-2021)

I got really nice band of PRMT5 Knock out and knockdown

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK 293
PRMT5 Antibody Western Blot validation (1:1000 dilution) in HEK 293 (Cat no:18436-1-AP)