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- KD/KO Validated
PSAT1 Polyclonal antibody
PSAT1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat and More (2)
Applications
WB, IP, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 10501-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, HEK-293 cells, HepG2 cells, K-562 cells, rat brain tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human pancreas cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 7 publications below |
| WB | See 53 publications below |
| IHC | See 7 publications below |
| IF | See 1 publications below |
| CoIP | See 2 publications below |
Product Information
10501-1-AP targets PSAT1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PSAT1 fusion protein Ag0776 |
| Full Name | phosphoserine aminotransferase 1 |
| Calculated Molecular Weight | 40 kDa |
| Observed Molecular Weight | 37-40 kDa |
| GenBank Accession Number | BC004863 |
| Gene Symbol | PSAT1 |
| Gene ID (NCBI) | 29968 |
| RRID | AB_2172597 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PSAT1, also named PSA and PSAT, belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family and SerC subfamily. It catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine. PSAT1 represents a new interesting target for CRC therapy. (PMID:18221502) It may be implicated in altered serine metabolism and schizophrenia spectrum conditions. (PMID:20955740)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PSAT1 antibody 10501-1-AP | Download protocol |
| IHC protocol for PSAT1 antibody 10501-1-AP | Download protocol |
| IF protocol for PSAT1 antibody 10501-1-AP | Download protocol |
| IP protocol for PSAT1 antibody 10501-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab Serine metabolism antagonizes antiviral innate immunity by preventing ATP6V0d2-mediated YAP lysosomal degradation. | ||
Nat Immunol PERK is a critical metabolic hub for immunosuppressive function in macrophages. | ||
Nat Commun Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence | ||
Nat Commun Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner. |
