Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 10501-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10501-1-AP (PSAT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10501-1-AP
WB result of PSAT1 antibody (10501-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSAT1 transfected HEK-293 cells.
WB analysis of Jurkat using 10501-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10501-1-AP (PSAT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10501-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10501-1-AP (PSAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10501-1-AP
IP result of anti-PSAT1 (IP:10501-1-AP, 3ug; Detection:10501-1-AP 1:300) with HeLa cells lysate 2480ug.
IHC staining of human pancreas cancer using 10501-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10501-1-AP (PSAT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10501-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10501-1-AP (PSAT1 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon cancer using 10501-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10501-1-AP (PSAT1 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HepG2 using 10501-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PSAT1 antibody (10501-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).
IF Staining of HepG2 using 10501-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PSAT1 antibody (10501-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
FC experiment of HepG2 using 10501-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PSAT1 Polyclonal antibody (10501-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

PSAT1 Polyclonal antibody

Name: PSAT1 Polyclonal antibody
Brand: Proteintech
SKU: 10501-1-AP

PSAT1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10501-1-AP

Publications(85)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

EC:2.6.1.52, EPIP, Phosphoserine aminotransferase, PSA, PSAT

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information
Publications (85)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, Jurkat cells, HEK-293 cells, HepG2 cells, K-562 cells, rat brain tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human pancreas cancer tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 10 publications below
WB	See 80 publications below
IHC	See 15 publications below
IF	See 4 publications below
CoIP	See 2 publications below

Product Information

10501-1-AP targets PSAT1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity	human, rat
Cited Reactivity	human, mouse, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0776 Product name: Recombinant human PSAT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-370 aa of BC004863 Sequence: MDAPRQVVNFGPGPAKLPHSVLLEIQKELLDYKGVGISVLEMSHRSSDFAKIINNTENLVRELLAVPDNYKVIFLQGGGCGQFSAVPLNLIGLKAGRCADYVVTGAWSAKAAEEAKKFGTINIVHPKLGSYTKIPDPSTWNLNPDASYVYYCANETVHGVEFDFIPDVKGAVLVCDMSSNFLSKPVDVSKFGVIFAGAQKNVGSAGVTVVIVRDDLLGFALRECPSVLEYKVQAGNSSLYNTPPCFSIYVMGLVLEWIKNNGGAAAMEKLSSIKSQTIYEIIDNSQGFYVCPVEPQNRSKMNIPFRIGNAKGDDALEKRFLDKALELNMLSLKGHRSVGGIRASLYNAVTIEDVQKLAAFMKKFLEMHQL Predict reactive species
Full Name	phosphoserine aminotransferase 1
Calculated Molecular Weight	40 kDa
Observed Molecular Weight	37-40 kDa
GenBank Accession Number	BC004863
Gene Symbol	PSAT1
Gene ID (NCBI)	29968
RRID	AB_2172597
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y617
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSAT1, also named PSA and PSAT, belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family and SerC subfamily. It catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine. PSAT1 represents a new interesting target for CRC therapy. (PMID:18221502) It may be implicated in altered serine metabolism and schizophrenia spectrum conditions. (PMID:20955740)

Protocols

Product Specific Protocols
IF protocol for PSAT1 antibody 10501-1-AP	Download protocol
IHC protocol for PSAT1 antibody 10501-1-AP	Download protocol
IP protocol for PSAT1 antibody 10501-1-AP	Download protocol
WB protocol for PSAT1 antibody 10501-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nature Stem cell function and stress response are controlled by protein synthesis. Authors - Sandra Blanco View Article
human	WB	Cell Metab Serine metabolism antagonizes antiviral innate immunity by preventing ATP6V0d2-mediated YAP lysosomal degradation. Authors - Long Shen View Article
mouse	WB	Nat Immunol PERK is a critical metabolic hub for immunosuppressive function in macrophages. Authors - Lydia N Raines View Article
mouse	WB	Cell Metab PMID: 28111214 Authors - Eric H Ma View Article
human	WB	Nat Commun Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence Authors - Yinsheng Wu View Article
human	WB	Nat Chem Biol PSAT1 impairs ferroptosis and reduces immunotherapy efficacy via GPX4 hydroxylation Authors - Peixiang Zheng View Article