CoraLite® Plus 488-conjugated PSAT1 Polyclonal antibody

Name: CoraLite® Plus 488-conjugated PSAT1 Polyclonal antibody, 100ul
Brand: Proteintech
SKU: CL488-10501-100UL

PSAT1 Polyclonal Antibody for FC (Intra)

Cat No. CL488-10501

Publications(1)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IF, FC (Intra)

Synonyms

EC:2.6.1.52, EPIP, Phosphoserine aminotransferase, PSA, PSAT

Formulation: PBS, Proclin300, BSA, Glycerol

PBS, Proclin300, BSA, Glycerol

Conjugate: CoraLite® Plus 488

Unconjugated CoraLite® Plus 488

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Filter: AllFC (Intra)

FC experiment of HepG2 using CL488-10501
1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human PSAT1 (CL488-10501) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive FC (Intra) detected in

HepG2 cells

Recommended dilution

Application	Dilution
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-10501 targets PSAT1 in IF, FC (Intra) applications and shows reactivity with human, rat samples.

Tested Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0776 Product name: Recombinant human PSAT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-370 aa of BC004863 Sequence: MDAPRQVVNFGPGPAKLPHSVLLEIQKELLDYKGVGISVLEMSHRSSDFAKIINNTENLVRELLAVPDNYKVIFLQGGGCGQFSAVPLNLIGLKAGRCADYVVTGAWSAKAAEEAKKFGTINIVHPKLGSYTKIPDPSTWNLNPDASYVYYCANETVHGVEFDFIPDVKGAVLVCDMSSNFLSKPVDVSKFGVIFAGAQKNVGSAGVTVVIVRDDLLGFALRECPSVLEYKVQAGNSSLYNTPPCFSIYVMGLVLEWIKNNGGAAAMEKLSSIKSQTIYEIIDNSQGFYVCPVEPQNRSKMNIPFRIGNAKGDDALEKRFLDKALELNMLSLKGHRSVGGIRASLYNAVTIEDVQKLAAFMKKFLEMHQL Predict reactive species
Full Name	phosphoserine aminotransferase 1
Calculated Molecular Weight	40 kDa
Observed Molecular Weight	37-40 kDa
GenBank Accession Number	BC004863
Gene Symbol	PSAT1
Gene ID (NCBI)	29968
RRID	AB_3672446
Conjugate	CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths	493 nm / 522 nm
Excitation Laser	Blue laser (488 nm)
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y617
Storage Buffer	PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage Conditions	Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PSAT1, also named PSA and PSAT, belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family and SerC subfamily. It catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine. PSAT1 represents a new interesting target for CRC therapy. (PMID:18221502) It may be implicated in altered serine metabolism and schizophrenia spectrum conditions. (PMID:20955740)