|Positive WB detected in||HeLa cells, HepG2 cells, human liver tissue|
|Western Blot (WB)||WB : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
17733-1-AP targets PURA in WB,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PURA fusion protein Ag12173|
|Full Name||purine-rich element binding protein A|
|Calculated molecular weight||322 aa, 35 kDa|
|Observed molecular weight||47 kDa|
|GenBank accession number||BC036087|
|Gene ID (NCBI)||5813|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PURA, also named as Transcriptional activator protein Pur-alpha, is a 322 amino acid protein, which belongs to the PUR DNA-binding protein family and can form homodimer or heterodimer with PURB and heterotrimer with PURB and YBX1/Y-box protein 1. PURA is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the MYC gene and may play a role in the initiation of DNA replication and in recombination. The calcualted molecular weight of PURA is 35 kDa, but modified PURA is 47 kDa. (PMID: 9716182)
Protective effects of astaxanthin on subarachnoid hemorrhage-induced early brain injury: Reduction of cerebral vasospasm and improvement of neuron survival and mitochondrial function.
Front Mol Neurosci
YAP and TAZ Regulate Cc2d1b and Purβ in Schwann Cells.
Nucleic Acids Res
Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation.