PURA Polyclonal antibody

PURA Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 17733-1-AP

Synonyms

PUR ALPHA, PUR1, PURA, PURALPHA



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, human liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

17733-1-AP targets PURA in WB,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PURA fusion protein Ag12173
Full Name purine-rich element binding protein A
Calculated molecular weight 322 aa, 35 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC036087
Gene symbol PURA
Gene ID (NCBI) 5813
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PURA, also named as Transcriptional activator protein Pur-alpha, is a 322 amino acid protein, which belongs to the PUR DNA-binding protein family and can form homodimer or heterodimer with PURB and heterotrimer with PURB and YBX1/Y-box protein 1. PURA is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the MYC gene and may play a role in the initiation of DNA replication and in recombination. The calcualted molecular weight of PURA is 35 kDa, but modified PURA is 47 kDa. (PMID: 9716182)

Protocols

Product Specific Protocols
WB protocol for PURA antibody 17733-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Acta Histochem

Protective effects of astaxanthin on subarachnoid hemorrhage-induced early brain injury: Reduction of cerebral vasospasm and improvement of neuron survival and mitochondrial function.

Authors - Yanbin Wang
ratWB

Front Mol Neurosci

YAP and TAZ Regulate Cc2d1b and Purβ in Schwann Cells.

Authors - Belin Sophie
humanWB

Nucleic Acids Res

Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation.

Authors - Ruibing Chen