Pan Acetylation Monoclonal antibody

Pan Acetylation Monoclonal Antibody for IF, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, Canine and More (2)

Applications

WB, IP, IF, CoIP, Acetylation assay, ELISA

Conjugate

Unconjugated

CloneNo.

1D5F2

Cat no : 66289-1-Ig

Synonyms

Acetylation, Acetylation Lysine, Pan Acetylation



Tested Applications

Positive WB detected inHeLa cells
Positive IF detected inHeLa cells, MDCK cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66289-1-Ig targets Pan Acetylation in WB, IP, IF, CoIP, Acetylation assay, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.

Tested Reactivity human, mouse, rat, Canine
Cited Reactivityhuman, mouse, rat, Drosophila, rice
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name Pan Acetylation
Observed Molecular Weight 17 kDa
Gene Symbol
Gene ID (NCBI)
RRIDAB_2881672
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Acetylation and De-acetylation are the important reaction in epigenetic. Many proteins, especially Histone, are with Acetylated of Lysine. This antibody is used to detect the Acetylation of Lysine. The 17 kDa in our detection is Acetylated Histone.

Protocols

Product Specific Protocols
WB protocol for Pan Acetylation antibody 66289-1-IgDownload protocol
IF protocol for Pan Acetylation antibody 66289-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Mol Cell

Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation

Authors - Chuanlong Wang
mouseWB,IF

Neuron

Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.

Authors - Xiaohui Li
DrosophilaWB

Dev Cell

HDAC6 Suppresses Age-Dependent Ectopic Fat Accumulation by Maintaining the Proteostasis of PLIN2 in Drosophila.

Authors - Yan Yan
humanWB

Cell Death Dis

RSL1D1 promotes the progression of colorectal cancer through RAN-mediated autophagy suppression.

Authors - Xunhua Liu
riceAcetylation assay

Plant Biotechnol J

The rice RNase P protein subunit Rpp30 confers broad-spectrum resistance to fungal and bacterial pathogens.

Authors - Wei Li
humanWB

Theranostics

EBV(LMP1)-induced metabolic reprogramming inhibits necroptosis through the hypermethylation of the RIP3 promoter.

Authors - Feng Shi