|Positive WB detected in||BxPC-3 cells, Neuro-2a cells, human placenta tissue, SGC-7901 cells, ROS1728 cells|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:2500-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Sample-dependent, check data in validation data gallery|
66491-1-Ig targets Periostin in WB, IHC,ELISA applications and shows reactivity with Human, Mouse, Rat samples.
|Tested Reactivity||Human, Mouse, Rat|
|Cited Reactivity||mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Periostin fusion protein Ag14487|
|Full Name||periostin, osteoblast specific factor|
|Calculated molecular weight||93 kDa|
|Observed molecular weight||85-90 kDa|
|GenBank accession number||BC106710|
|Gene ID (NCBI)||10631|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Periostin (POSTN, PN), originally named as osteoblast-specific factor 2 (OSF-2), is a 90-kDa secreted protein which is now classified as a matricellular protein. It is present in a wide variety of normal adult tissues and fetal tissues, and has a role in bone, tooth and heart development and function. Studies show that periostin is overexpressed in a broad range of human cancer types, including lung, ovary, breast and colon cancers. Recent evidence reveals that periostin is expressed by fibroblasts in the normal tissue and in the stroma of the primary tumour, and it is required to allow cancer stem cell maintenance. The isoforms of periostin are between 83 and 93 kDa in mass and differ in their C-terminal sequences, characterized by individual presence or absence of cassette exons 17-21 (UniProtKB/Swiss-Prot, PMID: 21997759).
Int J Mol Sci
Periostin Mediates Right Ventricular Failure through Induction of Inducible Nitric Oxide Synthase Expression in Right Ventricular Fibroblasts from Monocrotaline-Induced Pulmonary Arterial Hypertensive Rats.
Biochem Biophys Res Commun
Changed PGA and POSTN levels in choroid plexus are associated with depressive-like behaviors in mice.