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  • Featured Product
  • KD/KO Validated

RARS Polyclonal antibody

RARS Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27344-1-AP

Synonyms

Arginine tRNA ligase, arginyl tRNA synthetase, ArgRS, DALRD1, RARS

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RARS Polyclonal antibody (27344-1-AP)

    WB analysis of HeLa using 27344-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 27344-1-AP (RARS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RARS Polyclonal antibody (27344-1-AP)

    WB analysis of HeLa using 27344-1-AP

    WB result of RARS antibody (27344-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RARS transfected HeLa cells.

  • Western Blot (WB) analysis of U-251 cells using RARS Polyclonal antibody (27344-1-AP)

    WB analysis of U-251 using 27344-1-AP

    U-251 cells were subjected to SDS PAGE followed by western blot with 27344-1-AP (RARS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using RARS Polyclonal antibody (27344-1-AP)

    WB analysis of A431 using 27344-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 27344-1-AP (RARS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using RARS Polyclonal antibody (27344-1-AP)

    IP experiment of HeLa using 27344-1-AP

    IP result of anti-RARS (IP:27344-1-AP, 4ug; Detection:27344-1-AP 1:500) with HeLa cells lysate 3200 ug.

  • Immunohistochemistry (IHC) staining of human heart tissue using RARS Polyclonal antibody (27344-1-AP)

    IHC staining of human heart using 27344-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27344-1-AP (RARS antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using RARS Polyclonal antibody (27344-1-AP)

    IHC staining of human heart using 27344-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27344-1-AP (RARS antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RARS Polyclonal antibody (27344-1-AP)

    IHC staining of human breast cancer using 27344-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27344-1-AP (RARS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RARS Polyclonal antibody (27344-1-AP)

    IHC staining of human breast cancer using 27344-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27344-1-AP (RARS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, A431 cells, U-251 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman heart tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 27344-1-AP is RARS Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RARS fusion protein Ag26390
Full Name arginyl-tRNA synthetase
Calculated Molecular Weight 75 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC000528
Gene Symbol RARS
Gene ID (NCBI) 5917
RRIDAB_2880849
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RARS, also named ArgRS (arginyl-tRNA synthetase), belongs to the class-I aminoacyl-tRNA synthetase family which catalyze the aminoacylation of tRNA by their cognate amino acid. In higher eukaryotes, one of the two arginyl-tRNA synthetases (ArgRSs) has evolved to have an extended N-terminal domain that plays a crucial role in protein synthesis and cell growth and in integration into the multisynthetase complex (MSC) (PMID:25288775). RARS has two isoforms with the molecular weight of 75 and 67 kDa.

Protocols

Product Specific Protocols
WB protocol for RARS antibody 27344-1-APDownload protocol
IHC protocol for RARS antibody 27344-1-APDownload protocol
IP protocol for RARS antibody 27344-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

bioRxiv

Arginine limitation causes a directed DNA sequence evolution response in colorectal cancer cells

Authors - Dennis J Hsu
  • KD Validated
View Article
humanWB

Sci Adv

Arginine limitation drives a directed codon-dependent DNA sequence evolution response in colorectal cancer cells

Authors - Dennis J Hsu
  • KD Validated
View Article