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RARS Polyclonal antibody

RARS Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27344-1-AP

Synonyms

Arginine tRNA ligase, arginyl tRNA synthetase, ArgRS, DALRD1, RARS



Tested Applications

Positive WB detected inHeLa cells, A431 cells, U-251 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman heart tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 27344-1-AP is RARS Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RARS fusion protein Ag26390
Full Name arginyl-tRNA synthetase
Calculated molecular weight 75 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC000528
Gene symbol RARS
Gene ID (NCBI) 5917
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RARS, also named ArgRS (arginyl-tRNA synthetase), belongs to the class-I aminoacyl-tRNA synthetase family which catalyze the aminoacylation of tRNA by their cognate amino acid. In higher eukaryotes, one of the two arginyl-tRNA synthetases (ArgRSs) has evolved to have an extended N-terminal domain that plays a crucial role in protein synthesis and cell growth and in integration into the multisynthetase complex (MSC) (PMID:25288775). RARS has two isoforms with the molecular weight of 75 and 67 kDa.

Protocols

Product Specific Protocols
WB protocol for RARS antibody 27344-1-APDownload protocol
IHC protocol for RARS antibody 27344-1-APDownload protocol
IP protocol for RARS antibody 27344-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols