RB1 Antibody 18 Publications

Rabbit Polyclonal| Catalog number: 10048-2-Ig

Featured Product KD/KO validated

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Freight/Packing

Con: 150 μg/150 μl

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Species specificity:
human, mouse

Positive WB detected in:
Jurkat cells, A431 cells, Jurkat cells

Positive IP detected in:
A431 cells

Positive IHC detected in:
human prostate cancer tissue, human lung cancer tissue

Recommended dilution:
WB : 1:1000-1:6000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:200

Product Information


Source:
Rabbit

Purification method:
Protein A purification

Isotype:
IgG

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
retinoblastoma 1

Calculated molecular weight:
110 kDa

Observed molecular weight:
110 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
RB1

Synonyms
OSRC, p105 Rb, pp110, pRb, RB, RB1, retinoblastoma 1
Background

RB1, also named as pp110, pRb and p105 Rb, belongs to the retinoblastoma protein (RB) family. It is a key regulator of entry into cell division that acts as a tumor suppressor. RB1 acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. It is directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. It recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. RB1 controls histone H4 'Lys-20' trimethylation and inhibits the intrinsic kinase activity of TAF1. It mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity. This antibody is a rabbit polyclonal antibody raised against human RB1 fusion protein.


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