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  • KD/KO Validated

RHEB Polyclonal antibody

RHEB Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15924-1-AP

Synonyms

GTP binding protein Rheb, Ras homolog enriched in brain, RHEB, RHEB2



Tested Applications

Positive WB detected infetal human brain tissue, 3T3-L1 cells, DU 145 cells, HeLa cells, human brain tissue
Positive IHC detected inhuman brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15924-1-AP targets RHEB in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RHEB fusion protein Ag8747
Full Name Ras homolog enriched in brain
Calculated molecular weight 184 aa, 20 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC016155
Gene symbol RHEB
Gene ID (NCBI) 6009
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rheb is a member of the Ras superfamily small GTPases that plays important roles in the regulation of protein synthesis and growth in response to nutrient and growth factors. It is well known to bind and activate mTOR, a serine/threonine kinase which augments protein synthesis. Rheb has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity.

Protocols

Product Specific Protocols
WB protocol for RHEB antibody 15924-1-APDownload protocol
IHC protocol for RHEB antibody 15924-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Oncol

CircRNA-100338 Is Associated With mTOR Signaling Pathway and Poor Prognosis in Hepatocellular Carcinoma.

Authors - Xiu-Yan Huang
humanWB

Cell Physiol Biochem

Ox-LDL-Induced MicroRNA-155 Promotes Autophagy in Human Endothelial Cells via Repressing the Rheb/ mTOR Pathway.

Authors - Jinlin Lv
  • KD Validated
humanWB

Cell Death Dis

Synergistic antifibrotic effects of miR-451 with miR-185 partly by co-targeting EphB2 on hepatic stellate cells.

Authors - Xiaogang Chen
humanWB,IHC

Oncol Lett

Rheb phosphorylation is involved in p38-regulated/activated protein kinase-mediated tumor suppression in liver cancer.

Authors - Min Zheng
humanWB

Exp Mol Med

A brain somatic RHEB doublet mutation causes focal cortical dysplasia type II.

Authors - Shanshan Zhao
ratWB

Cell Metab

Crosstalk between glucocorticoid receptor and nutritional sensor mTOR in skeletal muscle.

Authors - Noriaki Shimizu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse brain