RNASEL Polyclonal antibody
RNASEL Polyclonal Antibody for WB,IHC,IF,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB,IF,IHC,ELISA
Conjugate
Unconjugated
Cat no : 22577-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | THP-1 cells, RAW 264.7 cells |
| Positive IHC detected in | human tonsillitis tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IF | See 1 publications below |
| ELISA | See 1 publications below |
Product Information
22577-1-AP targets RNASEL in WB,IF,IHC,ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RNASEL fusion protein Ag17409 |
| Full Name | ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent) |
| Calculated Molecular Weight | 741 aa, 84 kDa |
| Observed Molecular Weight | 84 kDa; 73 kDa |
| GenBank Accession Number | BC115697 |
| Gene Symbol | RNASEL |
| Gene ID (NCBI) | 6041 |
| RRID | AB_2631036 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RNASEL, also named as RNS4, is an endoribonuclease that functions in the interferon (IFN) antiviral response. RNASEL mediated apoptosis is the result of a JNK-dependent stress-response pathway leading to cytochrome c release from mitochondria and caspase-dependent apoptosis. There're some isoforms with calculated MW 84 kDa and 73 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RNASEL antibody 22577-1-AP | Download protocol |
| IHC protocol for RNASEL antibody 22577-1-AP | Download protocol |
| IF protocol for RNASEL antibody 22577-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) The CRISPR-Cas13a Gene-Editing System Induces Collateral Cleavage of RNA in Glioma Cells. | ||
Apoptosis The function of RNase L and its degradation mechanism in cardiac acute ischemic injury. | ||
Cardiovasc Diabetol Human serum RNase-L level is inversely associated with metabolic syndrome and age. | ||
iScience Inhibition of cGAS-Mediated Interferon Response Facilitates Transgene Expression. | ||
Cell Rep Deficiency of ER Ca2+ sensor STIM1 in AgRP neurons confers protection against dietary obesity. |
