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RPL3 Polyclonal antibody

RPL3 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 11005-1-AP

Synonyms

60S ribosomal protein L3, ribosomal protein L3, RPL3, TARBP B



Tested Applications

Positive WB detected inmouse placenta tissue, human kidney tissue, mouse pancreas tissue, Jurkat cells, HeLa cells, human placenta tissue, HEK-293 cells, multi-cells/tissue , mouse brain tissue, mouse lung tissue, mouse ovary tissue, K-562 cells, Raji cells, mouse kidney tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11005-1-AP targets RPL3 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL3 fusion protein Ag1436
Full Name ribosomal protein L3
Calculated molecular weight 46/27 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC012786
Gene symbol RPL3
Gene ID (NCBI) 6122
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ribosomes, the complexes that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL3, also named as TARBP-B and MGC104284, belongs to the ribosomal protein L3P family. This protein is a component of the 60S subunit. It can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. This is a rabbit polyclonal antibody raised against the C terminus of human RPL3.

Protocols

Product Specific Protocols
WB protocol for RPL3 antibody 11005-1-APDownload protocol
IHC protocol for RPL3 antibody 11005-1-APDownload protocol
IF protocol for RPL3 antibody 11005-1-APDownload protocol
IP protocol for RPL3 antibody 11005-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Aging (Albany NY)

RNA-seq analysis of the key long noncoding RNAs and mRNAs related to cognitive impairment after cardiac arrest and cardiopulmonary resuscitation.

Authors - Chan Chen
humanIHC

Carcinogenesis

DUOX2 promotes the progression of colorectal cancer cells by regulating the AKT pathway and interacting with RPL3.

Authors - Xue Zhang