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RPL3 Polyclonal antibody

RPL3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11005-1-AP

Synonyms

60S ribosomal protein L3, ribosomal protein L3, RPL3, TARBP B



Tested Applications

Positive WB detected inmouse placenta tissue, human kidney tissue, mouse pancreas tissue, Jurkat cells, HeLa cells, human placenta tissue, HEK-293 cells, multi-cells/tissue , mouse brain tissue, mouse lung tissue, mouse ovary tissue, K-562 cells, Raji cells, mouse kidney tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 1 publications below

Product Information

11005-1-AP targets RPL3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL3 fusion protein Ag1436
Full Name ribosomal protein L3
Calculated molecular weight 46/27 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC012786
Gene symbol RPL3
Gene ID (NCBI) 6122
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ribosomes, the complexes that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL3, also named as TARBP-B and MGC104284, belongs to the ribosomal protein L3P family. This protein is a component of the 60S subunit. It can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. This is a rabbit polyclonal antibody raised against the C terminus of human RPL3.

Protocols

Product Specific Protocols
WB protocol for RPL3 antibody 11005-1-APDownload protocol
IHC protocol for RPL3 antibody 11005-1-APDownload protocol
IF protocol for RPL3 antibody 11005-1-APDownload protocol
IP protocol for RPL3 antibody 11005-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Carcinogenesis

DUOX2 promotes the progression of colorectal cancer cells by regulating the AKT pathway and interacting with RPL3.

Authors - Xue Zhang