|Positive WB detected in||mouse placenta tissue, human kidney tissue, mouse pancreas tissue, Jurkat cells, HeLa cells, human placenta tissue, HEK-293 cells, multi-cells/tissue , mouse brain tissue, mouse lung tissue, mouse ovary tissue, K-562 cells, Raji cells, mouse kidney tissue|
|Positive IP detected in||mouse kidney tissue|
|Positive IHC detected in||human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11005-1-AP targets RPL3 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RPL3 fusion protein Ag1436|
|Full Name||ribosomal protein L3|
|Calculated molecular weight||46/27 kDa|
|Observed molecular weight||46 kDa|
|GenBank accession number||BC012786|
|Gene ID (NCBI)||6122|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Ribosomes, the complexes that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL3, also named as TARBP-B and MGC104284, belongs to the ribosomal protein L3P family. This protein is a component of the 60S subunit. It can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. This is a rabbit polyclonal antibody raised against the C terminus of human RPL3.
|Product Specific Protocols|
|WB protocol for RPL3 antibody 11005-1-AP||Download protocol|
|IHC protocol for RPL3 antibody 11005-1-AP||Download protocol|
|IF protocol for RPL3 antibody 11005-1-AP||Download protocol|
|IP protocol for RPL3 antibody 11005-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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