Uteroglobin/CC10 Polyclonal antibody

Uteroglobin/CC10 Polyclonal Antibody for FC (Intra), IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC (Intra), FC, ELISA

Conjugate

Unconjugated

Cat no : 10490-1-AP

Synonyms

CC10, CC16, CCPBP, CCSP, SCGB1A1, UGB, UP 1, UP1, Urinary protein 1, Urine protein 1, Uteroglobin, Uteroglobin/CC10



Tested Applications

Positive IHC detected inmouse lung tissue, human lung cancer tissue, rat lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse lung tissue, A549 cells
Positive FC detected inA549 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10490-1-AP targets Uteroglobin/CC10 in WB, IP, IHC, IF, FC (Intra), FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Uteroglobin/CC10 fusion protein Ag0758
Full Name secretoglobin, family 1A, member 1 (uteroglobin)
Calculated molecular weight 10 kDa
GenBank accession numberBC004481
Gene symbol SCGB1A1
Gene ID (NCBI) 7356
RRIDAB_2183285
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Uteroglobin, also known as CC10, is a member of the secretoglobin family of small secreted proteins. It is produced by the nonciliated, nonmucous, secretory epithelial clara cells of the pulmonary airways, was first identified in lung lavage by Singh and colleagues. It has been implicated in numerous functions including anti-inflammation, inhibition of phospholipase A2 and the sequestering of hydrophobic ligands. Defects in this gene are associated with a susceptibility to asthma.

Protocols

Product Specific Protocols
IHC protocol for Uteroglobin/CC10 antibody 10490-1-APDownload protocol
IF protocol for Uteroglobin/CC10 antibody 10490-1-APDownload protocol
FC protocol for Uteroglobin/CC10 antibody 10490-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Nature

SARS-CoV-2 Omicron variant replication in human bronchus and lung ex vivo.

Authors - Kenrie P Y Hui
humanIHC

Lancet Respir Med

Tropism, replication competence, and innate immune responses of the coronavirus SARS-CoV-2 in human respiratory tract and conjunctiva: an analysis in ex-vivo and in-vitro cultures.

Authors - Kenrie P Y Hui
humanIHC

Theranostics

A SCID mouse-human lung xenograft model of SARS-CoV-2 infection.

Authors - Wenkun Fu
humanIF

PLoS Pathog

Lung adenocarcinoma originates from retrovirus infection of proliferating type 2 pneumocytes during pulmonary post-natal development or tissue repair.

Authors - Murgia Claudio C
mouseIHC

J Pathol

Overexpression of the SARS-CoV-2 receptor ACE2 is induced by cigarette smoke in bronchial and alveolar epithelia.

Authors - Aibin Liu
humanWB

Antioxidants (Basel)

Empagliflozin-Enhanced Antioxidant Defense Attenuates Lipotoxicity and Protects Hepatocytes by Promoting FoxO3a- and Nrf2-Mediated Nuclear Translocation via the CAMKK2/AMPK Pathway.

Authors - Yangyang Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tessa (Verified Customer) (02-03-2019)

Used in 20pcw human fetal lung and got nice staining. Looks very specific with low background. Found that it did not require antigen retrieval in cryosections. In fact it turned out better in sections where 10mM Na Citrate buffer at pH6 was not used.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Human Fetal Lung