|Positive WB detected in||HeLa cells, HepG2 cells, NCI-H1299 cells, rat liver tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human breast cancer tissue, human lung cancer tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15235-1-AP targets SLC25A1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SLC25A1 fusion protein Ag7352|
|Full Name||solute carrier family 25 (mitochondrial carrier; citrate transporter), member 1|
|Calculated molecular weight||34 kDa|
|Observed molecular weight||30-34 kDa|
|GenBank accession number||BC004980|
|Gene ID (NCBI)||6576|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SLC25A1, also known as CIC or CTP, is a mitochondrial citrate transporter that exports citrate from the mitochondria to the cytosol. SLC25A1 is highly expressed in several tumor types. Recently it has been identified as a novel transcriptional target of mutant p53 and a negative tumor prognostic marker. In addition, defects in SLC25A1 has been found as a cause of combined D-2- and L-2-hydroxyglutaric aciduria. This antibody specifically recognized the endogenous SLC25A1; no protein was detected in cells from subjects containing two alleles with truncating mutations with this antibody. (24681808, 23561848)
|Product Specific Protocols|
|WB protocol for SLC25A1 antibody 15235-1-AP||Download protocol|
|IHC protocol for SLC25A1 antibody 15235-1-AP||Download protocol|
|IF protocol for SLC25A1 antibody 15235-1-AP||Download protocol|
|IP protocol for SLC25A1 antibody 15235-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Long non-coding RNA BRM promotes proliferation and invasion of papillary thyroid carcinoma by regulating the microRNA-331-3p/SLC25A1 axis.
J Inherit Metab Dis
An overview of combined D-2- and L-2-hydroxyglutaric aciduria: functional analysis of CIC variants.
J Biol Chem
A novel isoform of myosin 18A (Myo18Aγ) is an essential sarcomeric protein in mouse heart.
Cell Death Differ
Inhibition of the mitochondrial citrate carrier, Slc25a1, reverts steatosis, glucose intolerance, and inflammation in preclinical models of NAFLD/NASH.
Am J Hum Genet
Deficiency in SLC25A1, encoding the mitochondrial citrate carrier, causes combined D-2- and L-2-hydroxyglutaric aciduria.
Proline biosynthesis is a vent for TGFβ-induced mitochondrial redox stress.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Chunling (Verified Customer) (12-06-2018)
This antibody works great for western blot and Immunofluorescence. It is very specific with no non specific bands in western.