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  • KD/KO Validated

SLC25A1 Antibody

SLC25A1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15235-1-AP

Synonyms

CIC, Citrate transport protein, CTP, SLC20A3, SLC25A1, Tricarboxylate carrier protein



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, NCI-H1299 cells, rat liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15235-1-AP targets SLC25A1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC25A1 fusion protein Ag7352
Full Name solute carrier family 25 (mitochondrial carrier; citrate transporter), member 1
Calculated molecular weight 34 kDa
Observed molecular weight 30-34 kDa
GenBank accession numberBC004980
Gene symbol SLC25A1
Gene ID (NCBI) 6576
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC25A1, also known as CIC or CTP, is a mitochondrial citrate transporter that exports citrate from the mitochondria to the cytosol. SLC25A1 is highly expressed in several tumor types. Recently it has been identified as a novel transcriptional target of mutant p53 and a negative tumor prognostic marker. In addition, defects in SLC25A1 has been found as a cause of combined D-2- and L-2-hydroxyglutaric aciduria. This antibody specifically recognized the endogenous SLC25A1; no protein was detected in cells from subjects containing two alleles with truncating mutations with this antibody. (24681808, 23561848)

Protocols

Product Specific Protocols
WB protocol for SLC25A1 antibody 15235-1-APDownload protocol
IHC protocol for SLC25A1 antibody 15235-1-APDownload protocol
IF protocol for SLC25A1 antibody 15235-1-APDownload protocol
IP protocol for SLC25A1 antibody 15235-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncol Lett

Long non-coding RNA BRM promotes proliferation and invasion of papillary thyroid carcinoma by regulating the microRNA-331-3p/SLC25A1 axis.

Authors - Shihong Liu
humanWB

J Inherit Metab Dis

An overview of combined D-2- and L-2-hydroxyglutaric aciduria: functional analysis of CIC variants.

Authors - Ana Pop
  • KO Validated
humanWB

J Biol Chem

A novel isoform of myosin 18A (Myo18Aγ) is an essential sarcomeric protein in mouse heart.

Authors - Markus Horsthemke
  • KD Validated
human,mouseWB,IHC

Cell Death Differ

Inhibition of the mitochondrial citrate carrier, Slc25a1, reverts steatosis, glucose intolerance, and inflammation in preclinical models of NAFLD/NASH.

Authors - Mingjun Tan
humanWB

Am J Hum Genet

Deficiency in SLC25A1, encoding the mitochondrial citrate carrier, causes combined D-2- and L-2-hydroxyglutaric aciduria.

Authors - Benjamin Nota
mouseWB

EMBO J

Proline biosynthesis is a vent for TGFβ-induced mitochondrial redox stress.

Authors - Simon Schwörer

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Chunling (Verified Customer) (12-06-2018)

This antibody works great for western blot and Immunofluorescence. It is very specific with no non specific bands in western.

  • Applications: Western Blot,Immunofluorescence,
  • Cell Tissue Type: kidney cancer cells