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  • KD/KO Validated

SLC35A1 Polyclonal antibody

SLC35A1 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16342-1-AP

Synonyms

CMP SA Tr, CMP Sia Tr, CMP sialic acid transporter, CMPST, CST, hCST, inactive, SLC35A1



Tested Applications

Positive IHC detected inmouse spleen tissue, mouse testis tissue, human skin tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16342-1-AP targets SLC35A1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC35A1 fusion protein Ag9524
Full Name solute carrier family 35 (CMP-sialic acid transporter), member A1
Calculated Molecular Weight 337 aa, 37 kDa
Observed Molecular Weight 30-33 kDa
GenBank Accession NumberBC017807
Gene Symbol SLC35A1
Gene ID (NCBI) 10559
RRIDAB_2190077
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Solute carrier family 35 member A1 (SLC35A1) , also named as CMP-sialic acid transporter (CST) , is responsible for transferring CMP-sialic acid from the cytosol through Golgi membranes, thus playing a vital role in the terminal sialylation of glycan structures. Slc35a1 deficiency causes thrombocytopenia due to impaired megakaryocytopoiesis and excessive platelet clearance in the liver. In addition, SCL35A1 acts independently from sialic acid in functional α-DG O-mannosylation(PMID: 25552652).

Protocols

Product Specific Protocols
IHC protocol for SLC35A1 antibody 16342-1-APDownload protocol
IF protocol for SLC35A1 antibody 16342-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

J Exp Med

Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine.

Authors - Yalong Wang
humanWB, IF

Cells

Knockout of the CMP-Sialic Acid Transporter SLC35A1 in Human Cell Lines Increases Transduction Efficiency of Adeno-Associated Virus 9: Implications for Gene Therapy Potency Assays.

Authors - Antje Banning
  • KO Validated
humanWB

Cell Rep

A genome-wide CRISPR screen identifies UFMylation and TRAMP-like complexes as host factors required for hepatitis A virus infection.

Authors - Jessie Kulsuptrakul
  • KO Validated