|Positive WB detected in||HEK-293 cells, HepG2 cells, K-562 cells, HeLa cells, Jurkat cells, mouse testis tissue|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human kidney tissue, human ovary tissue, human skin tissue, human testis tissue, human heart tissue, human placenta tissue, human lung tissue, human spleen tissue, human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Hela cells, HepG2 cells, mouse brain tissue|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
11708-1-AP targets SMN in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SMN fusion protein Ag2260|
|Full Name||survival of motor neuron 2, centromeric|
|Calculated molecular weight||282 aa, 30 kDa|
|Observed molecular weight||38 kDa, 66-70 kDa|
|GenBank accession number||BC000908|
|Gene ID (NCBI)||6607|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of anterior horn cells in the spinal cord and concomitant symmetrical muscle weakness and atrophy (PMID: 16364894 ). SMA is caused by deletion or mutations of the survival motor neuron (SMN1) gene. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional (PMID: 17355180). A large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein (PMID: 10369862). The level of SMN protein correlates with phenotypic severity of SMA. This antibody, 11708-1-AP, raised against the recombinant full-length human SMN2 protein, recognizes all isoforms of SMN protein.
|Product Specific Protocols|
|WB protocol for SMN antibody 11708-1-AP||Download protocol|
|IHC protocol for SMN antibody 11708-1-AP||Download protocol|
|IF protocol for SMN antibody 11708-1-AP||Download protocol|
|IP protocol for SMN antibody 11708-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Assay Drug Dev Technol
Detection of survival motor neuron protein in buccal cells through electrochemiluminescence-based assay.
SMN Protein Can Be Reliably Measured in Whole Blood with an Electrochemiluminescence (ECL) Immunoassay: Implications for Clinical Trials.
Utility of survival motor neuron ELISA for spinal muscular atrophy clinical and preclinical analyses.
Evaluation of SMN protein, transcript, and copy number in the biomarkers for spinal muscular atrophy (BforSMA) clinical study.
Ann Clin Transl Neurol
Baseline results of the NeuroNEXT spinal muscular atrophy infant biomarker study.
Hum Mol Genet
Mild SMN missense alleles are only functional in the presence of SMN2 in mammals.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Rachel (Verified Customer) (08-19-2019)
Worked well in MSD immunoassays and cell based assays.