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  • KD/KO Validated

SMN Polyclonal antibody

SMN Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11708-1-AP

Synonyms

C BCD541, Component of gems 1, Gemin 1, SMN, SMN1, SMN1,SMN, SMN2, SMNC, SMNT, Survival motor neuron protein



Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, K-562 cells, HeLa cells, Jurkat cells, mouse testis tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue, human ovary tissue, human skin tissue, human testis tissue, human heart tissue, human placenta tissue, human lung tissue, human spleen tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells, HepG2 cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11708-1-AP targets SMN in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMN fusion protein Ag2260
Full Name survival of motor neuron 2, centromeric
Calculated molecular weight 282 aa, 30 kDa
Observed molecular weight 38 kDa, 66-70 kDa
GenBank accession numberBC000908
Gene symbol SMN2
Gene ID (NCBI) 6607
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of anterior horn cells in the spinal cord and concomitant symmetrical muscle weakness and atrophy (PMID: 16364894 ). SMA is caused by deletion or mutations of the survival motor neuron (SMN1) gene. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional (PMID: 17355180). A large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein (PMID: 10369862). The level of SMN protein correlates with phenotypic severity of SMA. This antibody, 11708-1-AP, raised against the recombinant full-length human SMN2 protein, recognizes all isoforms of SMN protein.

Protocols

Product Specific Protocols
WB protocol for SMN antibody 11708-1-APDownload protocol
IHC protocol for SMN antibody 11708-1-APDownload protocol
IF protocol for SMN antibody 11708-1-APDownload protocol
IP protocol for SMN antibody 11708-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
rat

Assay Drug Dev Technol

Detection of survival motor neuron protein in buccal cells through electrochemiluminescence-based assay.

Authors - Hannes Steinkellner

PLoS One

SMN Protein Can Be Reliably Measured in Whole Blood with an Electrochemiluminescence (ECL) Immunoassay: Implications for Clinical Trials.

Authors - Phillip Zaworski
human,mouseELISA

PLoS One

Utility of survival motor neuron ELISA for spinal muscular atrophy clinical and preclinical analyses.

Authors - Kobayashi Dione T DT
humanELISA

PLoS One

Evaluation of SMN protein, transcript, and copy number in the biomarkers for spinal muscular atrophy (BforSMA) clinical study.

Authors - Crawford Thomas O TO

Ann Clin Transl Neurol

Baseline results of the NeuroNEXT spinal muscular atrophy infant biomarker study.

Authors - Stephen J Kolb

Hum Mol Genet

Mild SMN missense alleles are only functional in the presence of SMN2 in mammals.

Authors - Chitra C Iyer

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Rachel (Verified Customer) (08-19-2019)

Worked well in MSD immunoassays and cell based assays.

  • Applications: Western Blot,Cell culture,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Fibroblasts