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SMN (Human,Mouse,Rat) Monoclonal antibody

SMN (Human,Mouse,Rat) Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3C10B5

Cat no : 60154-2-Ig

Synonyms

C BCD541, Component of gems 1, Gemin 1, SMN, SMN1, SMN1,SMN, SMN2, SMNC, SMNT, Survival motor neuron protein



Tested Applications

Positive WB detected inA375 cells, HeLa cells, HEK-293 cells, Jurkat cells
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

60154-2-Ig targets SMN (Human,Mouse,Rat) in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SMN (Human,Mouse,Rat) fusion protein Ag14333
Full Name survival of motor neuron 2, centromeric
Calculated molecular weight 282 aa, 30 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC000908
Gene symbol SMN2
Gene ID (NCBI) 6607
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The survival of motor neurons (SMN) genes are the disease genes of spinal muscular atrophy (SMA), a common motor neuron degenerative disease. The level of SMN protein correlates with phenotypic severity of SMA. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional, because a large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein. This antibody 60154-2-Ig can recognize human,mouse and rat SMN gene.

Protocols

Product Specific Protocols
WB protocol for SMN (Human,Mouse,Rat) antibody 60154-2-IgDownload protocol
IF protocol for SMN (Human,Mouse,Rat) antibody 60154-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols