SOD3 Antibody

SOD3 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 14316-1-AP

Synonyms

EC SOD, Extracellular superoxide dismutase, SOD3, Superoxide Dismutase 3



Tested Applications

Positive WB detected inhuman urine sample, human heart tissue, human urine, human placenta tissue
Positive IP detected inhuman placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Sample-dependent, check data in validation data gallery

Product Information

14316-1-AP targets SOD3 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SOD3 fusion protein Ag5556
Full Name superoxide dismutase 3, extracellular
Calculated molecular weight 26 kDa
Observed molecular weight 25-33 kDa
GenBank accession number BC014418
Gene symbol SOD3
Gene ID (NCBI) 6649
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SOD is located intracellular (SOD-1; cytosolic, SOD-2; mitochondrial matrix) and extracellular (SOD-3), and catalyzes the dismutation of superoxide anion to H2O2. SOD3, or EC-SOD, is the most recently characterized SOD, exists as a copper and zinc-containing tetramer, and is synthesized containing a signal peptide that directs this enzyme exclusively to extracellular spaces. SOD-3 is a Mn-containing tetrameric protein with a subunit Mr of 24-35 kDa. The activity of EC-SOD is dependent on its ability to form a tetramer of 135 kDa.

Protocols

Product Specific Protocols
WB protocol for SOD3 antibody 14316-1-APDownload protocol
IP protocol for SOD3 antibody 14316-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols