|Positive WB detected in||Jurkat cells, A431 cells, HEK-293 cells, K-562 cells|
|Positive IP detected in||A431 cells|
|Positive IHC detected in||human lung cancer tissue, human colon tissue, human stomach cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 21962-1-AP is SP1 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, monkey, mouse, rat, swine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SP1 fusion protein Ag16720|
|Full Name||Sp1 transcription factor|
|Calculated molecular weight||785 aa, 81 kDa|
|Observed molecular weight||95-106 kDa|
|GenBank accession number||BC062539|
|Gene ID (NCBI)||6667|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The transcription factor Sp1 is a C2H2 zinc-finger protein that is involved in the regulation of a wide variety of genes, including housekeeping genes and tumor-developing genes. It is associated with tumor development, growth, and metastasis [PMID: 16209919]. It regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Besides, it has a role in modulating the cellular response to DNA damage, recruiting SMARCA4/BRG1 on the c-FOS promoter, regulation of FE65 gene expression [PMID:11371615,16332679]. SP1 is detected with MW 95-105 kDa and 65 kDa in this paper(PMID: 10618488).
|Product Specific Protocols|
|WB protocol for SP1 antibody 21962-1-AP||Download protocol|
|IHC protocol for SP1 antibody 21962-1-AP||Download protocol|
|IF protocol for SP1 antibody 21962-1-AP||Download protocol|
|IP protocol for SP1 antibody 21962-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Int J Mol Sci
Silencing of Transcription Factor Sp1 Promotes SN1 Transporter Regulation by Ammonia in Mouse Cortical Astrocytes.
Involvement of multiple transcription factors in regulation of IL-β-induced MCP-1 expression in alveolar type II epithelial cells.
Int J Pharm
Long non-coding RNA FTH1P3 regulated metastasis and invasion of esophageal squamous cell carcinoma through SP1/NF-kB pathway.
J Diabetes Res
High Glucose Induces Endothelial COX2 and iNOS Expression via Inhibition of Monomethyltransferase SETD8 Expression.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Hannah (Verified Customer) (06-08-2020)
Single band at predicted size with with 2 minute exposure
David (Verified Customer) (01-15-2020)
Gave a single clear band at the expected molecular weight. Low protein input was used (10µg) for experimental reasons, hence the low antibody dilution required (1:100).