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SP1 Antibody

SP1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, chIP, ELISA

Conjugate

Unconjugated

Cat no : 21962-1-AP

Synonyms

SP1, Sp1 transcription factor, Transcription factor Sp1, TSFP1



Tested Applications

Positive WB detected inJurkat cells, A431 cells, HEK-293 cells, K-562 cells
Positive IP detected inA431 cells
Positive IHC detected inhuman lung cancer tissue, human colon tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21962-1-AP is SP1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, monkey, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SP1 fusion protein Ag16720
Full Name Sp1 transcription factor
Calculated molecular weight 785 aa, 81 kDa
Observed molecular weight 95-106 kDa
GenBank accession numberBC062539
Gene symbol SP1
Gene ID (NCBI) 6667
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The transcription factor Sp1 is a C2H2 zinc-finger protein that is involved in the regulation of a wide variety of genes, including housekeeping genes and tumor-developing genes. It is associated with tumor development, growth, and metastasis [PMID: 16209919]. It regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Besides, it has a role in modulating the cellular response to DNA damage, recruiting SMARCA4/BRG1 on the c-FOS promoter, regulation of FE65 gene expression [PMID:11371615,16332679]. SP1 is detected with MW 95-105 kDa and 65 kDa in this paper(PMID: 10618488).

Protocols

Product Specific Protocols
WB protocol for SP1 antibody 21962-1-APDownload protocol
IHC protocol for SP1 antibody 21962-1-APDownload protocol
IF protocol for SP1 antibody 21962-1-APDownload protocol
IP protocol for SP1 antibody 21962-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Int J Mol Sci

Silencing of Transcription Factor Sp1 Promotes SN1 Transporter Regulation by Ammonia in Mouse Cortical Astrocytes.

Authors - Katarzyna Dąbrowska
mouseWB

Mol Immunol

Involvement of multiple transcription factors in regulation of IL-β-induced MCP-1 expression in alveolar type II epithelial cells.

Authors - Chunguang Yan
ratWB

Biomed Pharmacother

PMID: 31060005

Authors - Zengxian Sun
humanWB

Int J Pharm

PMID: 29730175

Authors - Yao Cheng
humanWB

Biomed Pharmacother

Long non-coding RNA FTH1P3 regulated metastasis and invasion of esophageal squamous cell carcinoma through SP1/NF-kB pathway.

Authors - Lijun Yang
humanWB,IF,IP

J Diabetes Res

High Glucose Induces Endothelial COX2 and iNOS Expression via Inhibition of Monomethyltransferase SETD8 Expression.

Authors - Jie Qi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Hannah (Verified Customer) (06-08-2020)

Single band at predicted size with with 2 minute exposure

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: MRC5
FH

David (Verified Customer) (01-15-2020)

Gave a single clear band at the expected molecular weight. Low protein input was used (10µg) for experimental reasons, hence the low antibody dilution required (1:100).

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: SH-SY5Y