|Positive WB detected in||Jurkat cells|
|Positive IHC detected in||human ovary tumor tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11377-1-AP targets SP100 in WB, IHC, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||SP100 fusion protein Ag1939|
|Full Name||SP100 nuclear antigen|
|Calculated molecular weight||879 aa, 100 kDa|
|Observed molecular weight||100 kDa|
|GenBank accession number||BC011562|
|Gene ID (NCBI)||6672|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SP100 nuclear antigen, belongs to the nuclear bodies(NBs), has a role in the control of gene expression. It contains a HSR domain, which is important for the nuclear body targeting as well as for the dimerization, and one PxVxL motif, which if required for interaction with chromoshadow domains.
Role for a Filamentous Nuclear Assembly of IFI16, DNA, and Host Factors in Restriction of Herpesviral Infection.
THE VIRAL FGARAT HOMOLOG ORF75 OF RHESUS MONKEY RHADINOVIRUS EFFECTS PROTEASOMAL DEGRADATION OF THE ND10 COMPONENTS SP100 AND PML.
Kaposi's Sarcoma Associated Herpesvirus Tegument Protein ORF75 Is Essential for Viral Lytic Replication and Plays a Critical Role in the Antagonization of ND10-Instituted Intrinsic Immunity.