SP100 Polyclonal antibody

SP100 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11377-1-AP

Synonyms

Lysp100b, Nuclear autoantigen Sp 100, SP100, SP100 nuclear antigen, Speckled 100 kDa



Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11377-1-AP targets SP100 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SP100 fusion protein Ag1939
Full Name SP100 nuclear antigen
Calculated molecular weight 879 aa, 100 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC011562
Gene symbol SP100
Gene ID (NCBI) 6672
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SP100 nuclear antigen, belongs to the nuclear bodies(NBs), has a role in the control of gene expression. It contains a HSR domain, which is important for the nuclear body targeting as well as for the dimerization, and one PxVxL motif, which if required for interaction with chromoshadow domains.

Protocols

Product Specific Protocols
WB protocol for SP100 antibody 11377-1-APDownload protocol
IHC protocol for SP100 antibody 11377-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

MBio

Role for a Filamentous Nuclear Assembly of IFI16, DNA, and Host Factors in Restriction of Herpesviral Infection.

Authors - Philipp E Merkl

J Virol

THE VIRAL FGARAT HOMOLOG ORF75 OF RHESUS MONKEY RHADINOVIRUS EFFECTS PROTEASOMAL DEGRADATION OF THE ND10 COMPONENTS SP100 AND PML.

Authors - Alexander S Hahn
humanWB,IF

PLoS Pathog

Kaposi's Sarcoma Associated Herpesvirus Tegument Protein ORF75 Is Essential for Viral Lytic Replication and Plays a Critical Role in the Antagonization of ND10-Instituted Intrinsic Immunity.

Authors - Florian Full