P62/SQSTM1 Antibody 85 Publications

Rabbit Polyclonal| Catalog number: 18420-1-AP

Featured Product KD/KO validated

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Freight/Packing

Con: 44 μg/150 μl

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Species specificity:
human, mouse, rat

Positive WB detected in:
HEK-293 cells, HeLa cells, Jurkat cells

Positive IP detected in:
HEK-293 cells

Positive IHC detected in:
human gliomas tissue, human gliomas tissue, human heart tissue, human liver cancer tissue

Positive IF detected in:
Starvation treated HepG2 cells, A431 cells, HeLa cells, MCF-7 cells

Positive FC detected in:
HEK-293T cells

Recommended dilution:
WB : 1:1000-1:4000
IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
sequestosome 1

Calculated molecular weight:
48 kDa

Observed molecular weight:
62 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
SQSTM1

Synonyms
P62, sequestosome 1, SQSTM1, Ubiquitin binding protein p62
Background

Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and sometimes aggregated proteins for autophagic degradation. As a autophagy-specific substrate, p62 is degraded during the autophagic process, which makes intracellular level of p62 as a marker for autophagy flux. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer. p62 is a component of inclusion bodies/ protein aggregates found in human diseases, including Huntington's disease, Alzheimer's disease, Parkinson's disease in the brain, and nephropathic cystinosis in kidney (22074114, 22860231, 22714671). The molecular weight of p62 is predicted as 48/ 38 kDa, while western blot analyses using this antibody demonstrate the major band around 60-62 kDa in various tissues.


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