SRA1 Polyclonal antibody
SRA1 Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 24655-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A2780 cells, HEK-293 cells |
| Positive IHC detected in | human breast cancer tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | MCF-7 cells |
| Positive FC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 7 publications below |
| IHC | See 1 publications below |
Product Information
24655-1-AP targets SRA1 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SRA1 fusion protein Ag20082 |
| Full Name | steroid receptor RNA activator 1 |
| Calculated Molecular Weight | 26 kDa |
| Observed Molecular Weight | 30 kDa, 33 kDa |
| GenBank Accession Number | BC067895 |
| Gene Symbol | SRA1 |
| Gene ID (NCBI) | 10011 |
| RRID | AB_2879657 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Steroid receptor RNA activator 1 (SRA1), also known as seroid receptor RNA activator protein (SRAP) or SRA, is involved in modulating the activity of multiple transcription factors including the estrogen receptor (ER) (PMID: 20398657). It may play a role in tumorigenesis (PMID: 16152589). The SRA1 gene encodes both SRA1 protein and a non-coding functional RNA that functions as part of a ribonucleoprotein complex activating steroid receptor induced transcription. This polyclonal antibody recognizes endogenous SRA1 isoforms which migrate as a doublet on SDS-PAGE gels (PMID: 12565891; 23907597).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SRA1 antibody 24655-1-AP | Download protocol |
| IHC protocol for SRA1 antibody 24655-1-AP | Download protocol |
| IF protocol for SRA1 antibody 24655-1-AP | Download protocol |
| FC protocol for SRA1 antibody 24655-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Free Radic Biol Med Up-regulation of thioredoxin system by puerarin inhibits lipid uptake in macrophages | ||
Arterioscler Thromb Vasc Biol RP5-833A20.1/miR-382-5p/NFIA-Dependent Signal Transduction Pathway Contributes to the Regulation of Cholesterol Homeostasis and Inflammatory Reaction. | ||
J Lipid Res A lincRNA-DYNLRB2-2/GPR119/GLP-1R/ABCA1-dependent Signal Transduction Pathway Is Essential for the Regulation of Cholesterol Homeostasis and Inflammatory Reactions. | ||
J Lipid Res VNN1 Promotes Atherosclerosis Progression in apoE Deficiency Mice Fed a High Fat High Cholesterol Diet. | ||
PLoS One Dihydrocapsaicin Attenuates Plaque Formation through a PPARγ/LXRα Pathway in apoE(-/-) Mice Fed a High-Fat/High-Cholesterol Diet. | ||
J Ethnopharmacol Effect of Si-Miao-Yong-An decoction on the differentiation of monocytes, macrophages, and regulatory T cells in ApoE-/- mice. |
