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STAG1 Polyclonal antibody

STAG1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 14015-1-AP

Synonyms

Cohesin subunit SA 1, SA1, SCC3 homolog 1, STAG1, stromal antigen 1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human heart tissue using STAG1 Polyclonal antibody (14015-1-AP)

    WB analysis of human heart using 14015-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 14015-1-AP (STAG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using STAG1 Polyclonal antibody (14015-1-AP)

    WB analysis using 14015-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14015-1-AP (STAG1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using STAG1 Polyclonal antibody (14015-1-AP)

    WB analysis of Raji using 14015-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 14015-1-AP (STAG1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using STAG1 Polyclonal antibody (14015-1-AP)

    WB analysis of HeLa using 14015-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14015-1-AP (STAG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, human heart tissue, Raji cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

14015-1-AP targets STAG1 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STAG1 fusion protein Ag5123
Full Name stromal antigen 1
Calculated Molecular Weight 144 kDa
Observed Molecular Weight 155 kDa
GenBank Accession NumberBC064699
Gene Symbol STAG1
Gene ID (NCBI) 10274
RRIDAB_2194759
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STAG1, also named as SCC3 homolog 1, is a 1258 amino acid protein, which belongs to the SCC3 family. STAG1 interacts directly with RAD21 in cohesin complex and localizes in nucleus. STAG1 is a Component of cohesin complex, which is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. The calculate molecular weight of STAG1 is 144 kDa, but modified STAG1 is about 145-150 kDa.

Protocols

Product Specific Protocols
WB protocol for STAG1 antibody 14015-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Bone Res

Circular RNA circStag1 promotes bone regeneration by interacting with HuR.

Authors - Gaoyang Chen
View Article
humanWB

Proc Natl Acad Sci U S A

Genetic analysis of cancer drivers reveals cohesin and CTCF as suppressors of PD-L1.

Authors - Ena Oreskovic
View Article