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STAT1 Antibody 1 Publications

Mouse Monoclonal| Catalog number: 66545-1-Ig |CloneNo.: 1F7C6

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Tested Applications

Applications:
WB, IHC, CoIP, ELISA
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STAT1 Antibody WB HEK-293 cells 66545-1-Ig

Various cells were subjected to SDS PAGE followed by western blot with 66545-1-Ig (STAT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours

STAT1 Antibody IHC human ovary tumor tissue 66545-1-Ig

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66545-1-Ig (STAT1 antibody) at dilution of 1:400 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
Human, rat, mouse
Positive WB detected in:
HEK-293 cells, NIH/3T3 cells
Positive IHC detected in:
human ovary tumor tissue, human tonsillitis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:2000-1:10000
IHC : 1:200-1:800
Sample-dependent, check data in Validation tab

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2a
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
STAT1 fusion protein Ag0199
Full name:
signal transducer and activator of transcription 1, 91kDa
Calculated molecular weight:
83 kDa
Observed molecular weight:
84 kDa
GenBank accession number:
BC002704
Gene ID (NCBI):
6772
Gene symbol
STAT1
Synonyms
ISGF 3, STAT1, STAT91

Background

What is the molecular weight of STAT1? Are there any isoforms of STAT1? Is STAT1 post-translationally modified?

The molecular weight of STAT1 is 91 kDa for full-length STAT1α and 84 kDa for STAT1β, which is devoid of the C-terminal activation domain (PMID: 1502203). Phosphorylation of STAT1 regulates STAT1 activity. Phosphorylation of Tyr701 by Janus kinases (JAKs) triggers STAT1 homo- and hetero-dimerization with other STAT family members and also causes nuclear translocation, while phosphorylation of Ser727 is needed for full activation (PMID: 10851062). STAT1β lacks Ser727 and has therefore been considered to be inactive or acting in a dominant negative manner to STAT1α, although it has been further shown to be capable of transcription activation in vivo (PMID: 24710278).

 What is the subcellular localization of STAT1?

STAT1 is present in the cytoplasm and in the nucleus. Translocation of STAT1 from the cytoplasm to the nucleus is required for its regulation of gene expression and is dependent on phosphorylation (see above). However, low levels of unphosphorylated STAT1, often referred to as U-STAT1, can also be found in the nucleus, where U-STAT1 regulates the stability of heterochromatin by binding to chromatin in monomeric or dimeric states (PMID: 18840523).

 What is the tissue expression pattern of STAT1?

STAT1 is ubiquitously expressed.



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