|Positive WB detected in||K-562 cells, 3T3-L1 cells, DU 145 cells|
|Positive IP detected in||3T3-L1 cells|
|Positive IHC detected in||human breast cancer tissue, human cervical cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
13179-1-AP targets STAT5A in WB, IP, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||STAT5A fusion protein Ag3846|
|Full Name||signal transducer and activator of transcription 5A|
|Calculated molecular weight||794 aa, 92 kDa|
|Observed molecular weight||90-95 kDa, 50-55 kDa|
|GenBank accession number||BC027036|
|Gene ID (NCBI)||6776|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of STAT5A in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of human STAT5A is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of STAT5A in cells. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human STAT5A.
|Product Specific Protocols|
|WB protocol for STAT5A antibody 13179-1-AP||Download protocol|
|IHC protocol for STAT5A antibody 13179-1-AP||Download protocol|
|IP protocol for STAT5A antibody 13179-1-AP||Download protocol|
|FC protocol for STAT5A antibody 13179-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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