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- KD/KO Validated
STAT5A/B Polyclonal antibody
STAT5A/B Polyclonal Antibody for FC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB,IP,IHC,FC,ELISA
Conjugate
Unconjugated
Cat no : 13179-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Daudi cells, K-562 cells, DU 145 cells, 3T3-L1 cells, SGC-7901 cells |
| Positive IP detected in | 3T3-L1 cells |
| Positive IHC detected in | human cervical cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 17 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
Product Information
13179-1-AP targets STAT5A/B in WB,IP,IHC,FC,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, bovine, cow |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | STAT5A/B fusion protein Ag3846 |
| Full Name | signal transducer and activator of transcription 5A |
| Calculated Molecular Weight | 794 aa, 92 kDa |
| Observed Molecular Weight | 90-95 kDa, 50-55 kDa |
| GenBank Accession Number | BC027036 |
| Gene Symbol | STAT5A |
| Gene ID (NCBI) | 6776 |
| RRID | AB_2196760 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of STAT5A in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of human STAT5A is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of STAT5A in cells. This antibody is a rabbit polyclonal antibody raised against 445-794 aa of human STAT5A, the homology between this segment and the corresponding segment of human STAT5B is 92%. It shows that this antibody can cross-react with STAT5B.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for STAT5A/B antibody 13179-1-AP | Download protocol |
| IHC protocol for STAT5A/B antibody 13179-1-AP | Download protocol |
| IP protocol for STAT5A/B antibody 13179-1-AP | Download protocol |
| FC protocol for STAT5A/B antibody 13179-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Chemosphere MEHP promotes liver fibrosis by down-regulating STAT5A in BRL-3A hepatocytes.
| ||
Endocrinology A Novel Mechanism of hPRL-G129R, A Prolactin Antagonist, Inhibits Human Breast Cancer Cell Proliferation and Migration | ||
Front Oncol In-depth analysis of the expression and functions of signal transducers and activators of transcription in human ovarian cancer | ||
Ecotoxicol Environ Saf Mono-2-ethylhexyl phthalate (MEHP) promoted lipid accumulation via JAK2/STAT5 and aggravated oxidative stress in BRL-3A cells. | ||
J Cell Mol Med IL-27 Rα+ cells promoted allorejection via enhancing STAT1/3/5 phosphorylation. | ||
Cancer Cell Int LNK promotes the growth and metastasis of triple negative breast cancer via activating JAK/STAT3 and ERK1/2 pathway. |
