Syntaxin 3 Polyclonal antibody
Syntaxin 3 Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 15556-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | SH-SY5Y cells, human plasma tissue, Neuro-2a cells |
| Positive IP detected in | SH-SY5Y cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 10 publications below |
| IHC | See 2 publications below |
| IF | See 7 publications below |
| IP | See 3 publications below |
Product Information
15556-1-AP targets Syntaxin 3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Syntaxin 3 fusion protein Ag7925 |
| Full Name | syntaxin 3 |
| Calculated Molecular Weight | 33 kDa |
| Observed Molecular Weight | 33 kDa |
| GenBank Accession Number | BC007405 |
| Gene Symbol | STX3 |
| Gene ID (NCBI) | 6809 |
| RRID | AB_2198667 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Syntaxin-3 (also known as STX3) is a member of the syntaxin. Syntaxin-3 is a significant apical targeting protein in the epithelial membrane and in exocytosis process; it also acts as a vesicle transporter by cellular receptor in neutrophils, which is crucial for protein trafficking event (PMID: 25630102). It has been reported that syntaxin-3 has an important role in the growth of neurites, and serves as a direct target for omega-6 arachidonic acid (PMID: 1659826).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Syntaxin 3 antibody 15556-1-AP | Download protocol |
| IHC protocol for Syntaxin 3 antibody 15556-1-AP | Download protocol |
| IP protocol for Syntaxin 3 antibody 15556-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome. | ||
Mol Ther circMRPS35 promotes malignant progression and cisplatin resistance in hepatocellular carcinoma | ||
PLoS Genet BBSome function is required for both the morphogenesis and maintenance of the photoreceptor outer segment. | ||
Hum Mol Genet Photoreceptor cilia, in contrast to primary cilia, grant entry to a partially assembled BBSome. | ||
Front Mol Neurosci Farnesylation of the Transducin G Protein Gamma Subunit Is a Prerequisite for Its Ciliary Targeting in Rod Photoreceptors. | ||
J Biol Chem Glia Maturation Factor-γ Regulates Monocyte Migration through Modulation of β1-Integrin. |
