Syntaxin 2 Polyclonal antibody

• Featured Product
• KD/KO Validated

Syntaxin 2 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 55033-1-AP

Synonyms

EPIM, Epimorphin, EPM, STX2, STX2A, STX2B, STX2C, syntaxin 2

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Validation Data Gallery

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  WB analysis of A431 using 55033-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 55033-1-AP (Syntaxin 2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 55033-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55033-1-AP (Syntaxin 2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55033-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55033-1-AP (Syntaxin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 55033-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 55033-1-AP (Syntaxin 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

55033-1-AP targets Syntaxin 2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: syntaxin 2
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: NM_001980
• Gene Symbol: STX2
• Gene ID (NCBI): 2054
• RRID: AB_11182166
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Syntaxin 2 (STX2), also named as EPIM, STX2A, STX2B and STX2C, belongs to the syntaxin family. It is essential for epithelial morphogenesis. STX2 is a member of the SNARE membrane fusion machinery which localized to the midbody during cytokinesis in rat and canine kidney cell lines. Inhibition of STX2 and Vamp8 function by overexpression of nonmembrane-anchored mutants caused failure of cytokinesis leading to the formation of binucleated cells. Time-lapse microscopy showed that only midbody abscission and not further upstream events, such as furrowing, were affected. The antibody is specific to STX2.

Publications

Species	Application	Title
human	WB,IHC,IF	Cell Death Dis; STX2 promotes colorectal cancer metastasis through a positive feedback loop that activates the NF-κB pathway.; Authors - Yongxia Wang ; KD Validated
human	WB	J Cell Sci; Syntaxin 7 contributes to breast cancer cell invasion by promoting invadopodia formation.; Authors - Sameena Parveen
human	WB,IHC	Life Sci; STX2 drives colorectal cancer proliferation via upregulation of EXOSC4.; Authors - Yong-Xia Wang ; KD Validated
human	IHC, WB	J Cancer; Syntaxin 2 promotes colorectal cancer growth by increasing the secretion of exosomes.; Authors - Yongxia Wang
rat	WB	Neuroscience; SNAP25/syntaxin4/VAMP2/Munc18-1 Complexes in Spinal Dorsal Horn Contributed to Inflammatory Pain.; Authors - Xing-Lian Duan