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- KD/KO Validated
Syntaxin 1A / Syntaxin 1B Monoclonal antibody
Syntaxin 1A / Syntaxin 1B Monoclonal Antibody for IHC, WB,ELISA
Cat no : 66437-1-Ig
|Positive WB detected in||Y79 cells, fetal human brain tissue, rat brain tissue|
|Positive IHC detected in||human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66437-1-Ig targets Syntaxin 1A / Syntaxin 1B in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Syntaxin 1A / Syntaxin 1B fusion protein Ag10257|
|Full Name||syntaxin 1B|
|Calculated molecular weight||288 aa, 33 kDa|
|Observed molecular weight||33 kDa|
|GenBank accession number||BC062298|
|Gene ID (NCBI)||112755|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Syntaxins are a family of transmembrane proteins that belong to the SNARE complex (PMID: 11737951). In conjunction with other SNAREs and with the cytoplasmic NSF and SNAP proteins, syntaxins mediate vesicle fusion in diverse vesicular transport processes along the exocytic and the endocytic pathway (PMID: 11737951). Syntaxin 1A and 1B, two closely related isoforms of syntaxin 1, are involved in synaptic vesicle docking and fusion during neurotransmitter release (PMID: 7690687; 1321498; 18691641). This antibody raised against 1-263 aa of human syntaxin 1B detects a ~33 kDa band of syntaxin 1 and an additional band of 55-60 kDa, which probably represents a syntaxin 1 dimer (PMID: 10100611).
Munc18 and Munc13 serve as a functional template to orchestrate neuronal SNARE complex assembly.
Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.