• Featured Product
  • KD/KO Validated

Syntaxin 1A / Syntaxin 1B Monoclonal antibody

Syntaxin 1A / Syntaxin 1B Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2D3B5

Cat no : 66437-1-Ig

Synonyms

STX1B, STX1B1, STX1B2, Syntaxin 1A / Syntaxin 1B, syntaxin 1B, Syntaxin 1B1, Syntaxin 1B2



Tested Applications

Positive WB detected inY79 cells, fetal human brain tissue, rat brain tissue
Positive IHC detected inrat brain tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy Syntaxin 1A/1B Ready-To-Use IHC Kit. Syntaxin 1A/1B primary antibody included.
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66437-1-Ig targets Syntaxin 1A / Syntaxin 1B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Syntaxin 1A / Syntaxin 1B fusion protein Ag10257
Full Name syntaxin 1B
Calculated molecular weight 288 aa, 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC062298
Gene symbol STX1B
Gene ID (NCBI) 112755
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Syntaxins are a family of transmembrane proteins that belong to the SNARE complex (PMID: 11737951). In conjunction with other SNAREs and with the cytoplasmic NSF and SNAP proteins, syntaxins mediate vesicle fusion in diverse vesicular transport processes along the exocytic and the endocytic pathway (PMID: 11737951). Syntaxin 1A and 1B, two closely related isoforms of syntaxin 1, are involved in synaptic vesicle docking and fusion during neurotransmitter release (PMID: 7690687; 1321498; 18691641). This antibody raised against 1-263 aa of human syntaxin 1B detects a ~33 kDa band of syntaxin 1 and an additional band of 55-60 kDa, which probably represents a syntaxin 1 dimer (PMID: 10100611).

Protocols

Product Specific Protocols
WB protocol for Syntaxin 1A / Syntaxin 1B antibody 66437-1-IgDownload protocol
IHC protocol for Syntaxin 1A / Syntaxin 1B antibody 66437-1-IgDownload protocol
IF protocol for Syntaxin 1A / Syntaxin 1B antibody 66437-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Nat Commun

Munc18 and Munc13 serve as a functional template to orchestrate neuronal SNARE complex assembly.

Authors - Shen Wang
humanWB,IF

Brain Commun

Transcriptomic analysis of human brains with Alzheimer's disease reveals the altered expression of synaptic genes linked to cognitive deficits.

Authors - Jamal B Williams
mouseWB

Clin Sci (Lond)

Chronic hepatitis C virus infection impairs insulin secretion by regulation of p38δ MAPK-dependent exocytosis in pancreatic β-cells.

Authors - Jizheng Chen
mouseWB,IF

Nat Commun

Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.

Authors - I-Ling Lu
  • KD Validated