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Syntaxin 1A / Syntaxin 1B Monoclonal antibody
Syntaxin 1A / Syntaxin 1B Monoclonal Antibody for IF, IHC, WB, ELISA
Cat no : 66437-1-Ig
Validation Data Gallery
|Positive WB detected in||Y79 cells, fetal human brain tissue, rat brain tissue|
|Positive IHC detected in||rat brain tissue, human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse brain tissue|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunohistochemistry (IHC)||IHC : 1:2000-1:8000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
66437-1-Ig targets Syntaxin 1A / Syntaxin 1B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Syntaxin 1A / Syntaxin 1B fusion protein Ag10257|
|Full Name||syntaxin 1B|
|Calculated molecular weight||288 aa, 33 kDa|
|Observed molecular weight||33 kDa|
|GenBank accession number||BC062298|
|Gene ID (NCBI)||112755|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Syntaxins are a family of transmembrane proteins that belong to the SNARE complex (PMID: 11737951). In conjunction with other SNAREs and with the cytoplasmic NSF and SNAP proteins, syntaxins mediate vesicle fusion in diverse vesicular transport processes along the exocytic and the endocytic pathway (PMID: 11737951). Syntaxin 1A and 1B, two closely related isoforms of syntaxin 1, are involved in synaptic vesicle docking and fusion during neurotransmitter release (PMID: 7690687; 1321498; 18691641). This antibody raised against 1-263 aa of human syntaxin 1B detects a ~33 kDa band of syntaxin 1 and an additional band of 55-60 kDa, which probably represents a syntaxin 1 dimer (PMID: 10100611).
|Product Specific Protocols|
|WB protocol for Syntaxin 1A / Syntaxin 1B antibody 66437-1-Ig||Download protocol|
|IHC protocol for Syntaxin 1A / Syntaxin 1B antibody 66437-1-Ig||Download protocol|
|IF protocol for Syntaxin 1A / Syntaxin 1B antibody 66437-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
Munc18 and Munc13 serve as a functional template to orchestrate neuronal SNARE complex assembly.
Transcriptomic analysis of human brains with Alzheimer's disease reveals the altered expression of synaptic genes linked to cognitive deficits.
Clin Sci (Lond)
Chronic hepatitis C virus infection impairs insulin secretion by regulation of p38δ MAPK-dependent exocytosis in pancreatic β-cells.
Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.