SNAP23 Polyclonal antibody

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• SNAP23 Polyclonal antibody (10825-1-AP)

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• KD/KO Validated

SNAP23 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 10825-1-AP Copied!

Publications (35)

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IHC-Autostainer, IF, IP, CoIP, ELISA

Synonyms

HsT17016, SNAP 23, SNAP-23, SNAP23A, SNAP23B

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  WB analysis using 10825-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10825-1-AP (SNAP23 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 10825-1-AP

  IP result of anti-SNAP23 (IP:10825-1-AP, 4ug; Detection:10825-1-AP 1:500) with HeLa cells lysate 880ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 10825-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10825-1-AP (SNAP23 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human prostate cancer using 10825-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10825-1-AP (SNAP23 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Raji cells, SKOV-3 cells, human placenta tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human placenta tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10825-1-AP targets SNAP23 in WB, IHC, IHC-Autostainer, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1269

  Product name: Recombinant human SNAP23 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-211 aa of BC000148

  Sequence: MDNLSSEEIQQRAHQITDESLESTRRILGLAIESQDAGIKTITMLDEQKEQLNRIEEGLDQINKDMRETEKTLTELNKCCGLCVCPCNRTKNFESGKAYKTTWGDGGENSPCNVVSKQPGPVTNGQLQQPTTGAASGGYIKRITNDAREDEMEENLTQVGSILGNLKDMALNIGNEIDAQNPQIKRITDKADTNRDRIDIANARAKKLIDS

• Full Name: synaptosomal-associated protein, 23kDa
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 23-25 kDa
• GenBank Accession Number: BC000148
• Gene Symbol: SNAP23
• Gene ID (NCBI): 8773
• RRID: AB_2192022
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O00161
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SNAP23 (synaptosome-associated protein 23 kDa) is a widely expressed SNARE protein that mediates vesicle docking and membrane fusion during exocytosis. SNAP23 plays a crucial role in insulin-regulated glucose metabolism, cell division, and cell proliferation.

Publications

What published studies show

The SNAP23 Polyclonal antibody (Cat# 10825-1-AP) has 35 citations spanning journals including Immunity, Blood, Nature Communications, Cell Death & Differentiation, Oncogene, J Cell Biol, Adv Sci, Cell Mol Life Sci, and J Exp Clin Cancer Res. Research fields covered include sepsis and thrombosis, exosome secretion, SNARE-mediated vesicle trafficking, colorectal and hepatocellular cancer, insulin secretion, neurodegeneration, mast cell biology, lipid metabolism, and gene therapy vector production.

Automatically generated from the 35 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	WB	Immunity; STING activation in platelets aggravates septic thrombosis by enhancing platelet activation and granule secretion; Authors - Mina Yang
mouse	WB	Blood; Platelet NLRP6 protects against microvascular thrombosis in sepsis; Authors - Huimin Jiang
human	WB,IF	J Extracell Vesicles; Identification of the SNARE complex that mediates the fusion of multivesicular bodies with the plasma membrane in exosome secretion; Authors - Chuqi Liu ; KO Validated
human	WB,IF	Nat Commun; KRAS regulation by small non-coding RNAs and SNARE proteins.; Authors - Yonglu Che
mouse	IHC	Nat Commun; SNAP-23 mediated vesicular trafficking in oligodendrocytes is necessary to maintain adult myelin integrity in mice.; Authors - Chun Hin Chow ; KO Validated
human	WB	J Exp Clin Cancer Res; Let-7a regulates EV secretion and mitochondrial oxidative phosphorylation by targeting SNAP23 in colorectal cancer.; Authors - You Dong Liu ; KD Validated

Reviews

5.0 out of 5

1 verified customer reviews

What customers say

The single review for this product is a 5-star rating. The reviewer reports that the antibody recognizes the correct band size and performs well in Western Blot applications. Overall, the feedback is positive, highlighting reliable specificity and strong performance for WB use.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Shweta (Verified Customer) (11-18-2024)

Antibody recognizes the correct band size and works great in WB

• Applications: Western Blot