Syntaxin 4 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• Syntaxin 4 Polyclonal antibody (14988-1-AP)

• Featured Product
• KD/KO Validated

Syntaxin 4 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 14988-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

Syntaxin-4, STX4A, STX4, Renal carcinoma antigen NY-REN-31

• 

  WB analysis using 14988-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14988-1-AP (Syntaxin 4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 14988-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14988-1-AP (Syntaxin 4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 14988-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14988-1-AP (Syntaxin 4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat kidney using 14988-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 14988-1-AP (Syntaxin 4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse kidney using 14988-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14988-1-AP (Syntaxin 4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse kidney using 14988-1-AP

  IP result of anti-Syntaxin 4 (IP:14988-1-AP, 4ug; Detection:14988-1-AP 1:1500) with mouse kidney tissue lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach using 14988-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 14988-1-AP (Syntaxin 4 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

---

Tested Applications

• Positive WB detected in: mouse kidney tissue, rat kidney tissue, HEK-293 cells, Jurkat cells
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14988-1-AP targets Syntaxin 4 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6973

  Product name: Recombinant human STX4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-272 aa of BC002436

  Sequence: MRDRTHELRQGDDSSDEEDKERVALVVHPGTARLGSPDEEFFHKVRTIRQTIVKLGNKVQELEKQQVTILATPLPEESMKQELQNLRDEIKQLGREIRLQLKAIEPQKEEADENYNSVNTRMRKTQHGVLSQQFVELINKCNSMQSEYREKNVERIRRQLKITNAGMVSDEELEQMLDSGQSEVFVSNILKDTQVTRQALNEISARHSEIQQLERSIRELHDIFTFLATEVEMQGEMINRIEKNILSSADYVERGQEHVKTALENQKKARKK

• Full Name: syntaxin 4
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 35-37 kDa
• GenBank Accession Number: BC002436
• Gene Symbol: STX4
• Gene ID (NCBI): 6810
• RRID: AB_2286910
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q12846
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Brain; Reduced oligodendrocyte exosome secretion in multiple system atrophy involves SNARE dysfunction.; Authors - Zhenwei Yu
human	WB	Front Immunol; Bi-Allelic Mutations in STXBP2 Reveal a Complementary Role for STXBP1 in Cytotoxic Lymphocyte Killing.; Authors - Jamie A Lopez
human	WB	Int J Biol Macromol; PKCε controls the fusion of secretory vesicles in mast cells in a phosphatidic acid-dependent mode.; Authors - Emilio M Serrano-López
human	WB	Br J Pharmacol; CRP promotes atherosclerosis by increasing LDL transcytosis across endothelial cells.; Authors - Fang Bian
human	WB	J Biol Chem; Glia Maturation Factor-γ Regulates Monocyte Migration through Modulation of β1-Integrin.; Authors - Wulin Aerbajinai
human	WB	J Transl Med; Angiopoietin2-mediated caveolin1 phosphorylation regulating transcytosis of renal tubular epithelial cell contributes to the occurrence of albuminuria under high glucose exposure.; Authors - Jing Liu