STXBP2 Polyclonal antibody

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• STXBP2 Polyclonal antibody (15312-1-AP)

STXBP2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15312-1-AP Copied!

Publications (7)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

Hunc18b, MUNC18 2, Munc18-2, pp10122, Protein unc-18 homolog 2

• 

  WB analysis using 15312-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15312-1-AP (STXBP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 15312-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15312-1-AP (STXBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse kidney using 15312-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15312-1-AP (STXBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat kidney using 15312-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 15312-1-AP (STXBP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 15312-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 15312-1-AP (STXBP2 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 15312-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 15312-1-AP (STXBP2 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 15312-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15312-1-AP (STXBP2 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 15312-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15312-1-AP (STXBP2 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse brain tissue, mouse kidney tissue, K-562 cells
• Positive IHC detected in: human lung cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15312-1-AP targets STXBP2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7537

  Product name: Recombinant human STXBP2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 241-590 aa of BC002869

  Sequence: LHELTFQAMAYDLLDIEQDTYRYETTGLSEAREKAVLLDEDDDLWVELRHMHIADVSKKVTELLRTFCESKRLTTDKANIKDLSQILKKMPQYQKELNKYSTHLHLADDCMKHFKGSVEKLCSVEQDLAMGSDAEGEKIKDSMKLIVPVLLDAAVPAYDKIRVLLLYILLRNGVSEENLAKLIQHANVQAHSSLIRNLEQLGGTVTNPGGSGTSSRLEPRERMEPTYQLSRWTPVIKDVMEDAVEDRLDRNLWPFVSDPAPTASSQAAVSARFGHWHKNKAGIEARAGPRLIVYVMGGVAMSEMRAAYEVTRATEGKWEVLIGSSHILTPTRFLDDLKALDKKLEDIALP

• Full Name: syntaxin binding protein 2
• Calculated Molecular Weight: 593 aa, 66 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC002869
• Gene Symbol: STXBP2
• Gene ID (NCBI): 6813
• RRID: AB_2196821
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15833
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Syntaxin-binding protein 2 (STXBP2, also known as Unc18-2) belongs to the STXBP/unc-18/SEC1 family. STXBP2 is involved in intracellular trafficking, control of SNARE (soluble NSF attachment protein receptor) complex assembly, and the release of cytotoxic granules by natural killer cells. Mutations in the STXBP2 gene are associated with familial hemophagocytic lymphohistiocytosis.

Publications

What published studies show

The STXBP2 Polyclonal antibody (Cat# 15312-1-AP) has 7 citations published in Nanomicro Lett, Journal of Experimental Medicine, Frontiers in Immunology, Journal of Cell Biology, Journal of Inflammation Research, Neuroscience, and International Journal of Hematology. Research fields span intracellular trafficking and SNARE complex biology, primary immunodeficiency, familial hemophagocytic lymphohistiocytosis, natural killer cell cytotoxicity, sepsis-induced lung injury, inflammatory pain mechanisms, and nanoparticle delivery monitoring.

Automatically generated from the 7 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	IF	Nanomicro Lett; Molecular Mechanisms of Intracellular Delivery of Nanoparticles Monitored by an Enzyme-Induced Proximity Labeling; Authors - Junji Ren
human	WB	J Exp Med; Transcriptional regulation of Munc13-4 expression in cytotoxic lymphocytes is disrupted by an intronic mutation associated with a primary immunodeficiency.; Authors - Frank Cichocki
human	WB	Front Immunol; An N-Terminal Missense Mutation in STX11 Causative of FHL4 Abrogates Syntaxin-11 Binding to Munc18-2.; Authors - Martha-Lena Müller
human	WB	J Cell Biol; The septin cytoskeleton regulates natural killer cell lytic granule release.; Authors - Prasad V Phatarpekar
human	WB	J Inflamm Res; Identification of Biomarkers for Sepsis-Induced Acute Lung Injury Through Bioinformatics and Machine Learning Approaches, with Experimental Validation.; Authors - Yannian Luo
rat	WB	Neuroscience; SNAP25/syntaxin4/VAMP2/Munc18-1 Complexes in Spinal Dorsal Horn Contributed to Inflammatory Pain.; Authors - Xing-Lian Duan