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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated SUMO2/3 Monoclonal antibody

SUMO2/3 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1A1B3

Cat no : CL594-67154

Synonyms

HSMT3, Sentrin 2, SMT3 homolog 2, SMT3B, SMT3H2, SUMO 2, SUMO 3, SUMO2, SUMO2/3, Ubiquitin like protein SMT3B

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated SUMO2/3 Monoclonal antibod (CL594-67154)

    IF Staining of HeLa using CL594-67154

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CL594-67154 (SUMO2/3 antibody) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated SUMO2/3 Monoclonal antibod (CL594-67154)

    IF Staining of HeLa using CL594-67154

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 SUMO2/3 antibody (CL594-67154, Clone: 1A1B3 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated SUMO2/3 Monoclonal antibod (CL594-67154)

    FC experiment of HeLa using CL594-67154

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human SUMO2/3 (CL594-67154, Clone:1A1B3) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated SUMO2/3 Monoclonal antibod (CL594-67154)

    FC experiment of HeLa using CL594-67154

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human SUMO2/3 (CL594-67154, Clone:1A1B3) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67154 targets SUMO2/3 in IF, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SUMO2/3 fusion protein Ag28672
Full Name SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae)
Calculated Molecular Weight 11 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC008450
Gene Symbol SUMO2
Gene ID (NCBI) 6613
RRIDAB_2883641
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ubiquitin is most famous for its function in targeting proteins for degradation by the 26S proteasome, ubiquitin needs to be attached to a substrate in chains (polyubiquitylation) before being recognized by proteasome. Similarly, SUMO (small ubiquitin-related modifier) can be linked to substrates in chains (polysumoylation), SUMO modification has been implicated in many important cellular processes including the control of genome stability, signal transduction, targeting to and formation of nuclear compartments, cell cycle and meiosis. There are 4 confirmed SUMO isoforms in human, SUMO-1, SUMO-2, SUMO-3 and SUMO-4. SUMO-2 and SUMO-3 are nearly identical but are distinct from SUMO-1. SUMO2/3 conjugation was recently widely involved in neuroprotective activities. A substitution (M55V) of SUMO4 was strongly associated with the pathogenesis of type 1 diabetes (T1D) involving NF kappa B related mechanisms.

Protocols

Product Specific Protocols
IF protocol for CL594 SUMO2/3 antibody CL594-67154Download protocol
FC protocol for CL594 SUMO2/3 antibody CL594-67154Download protocol
Standard Protocols
Click here to view our Standard Protocols