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Transgelin 2 Antibody

Transgelin 2 Monoclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2E5G1

Cat no : 60044-1-Ig

Synonyms

SM22 beta, SM22beta, TAGLN2, Transgelin 2, Transgelin 2/TAGLN2



Tested Applications

Positive WB detected inK562 cells, K-562 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

60044-1-Ig targets Transgelin 2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Transgelin 2 fusion protein Ag0337
Full Name transgelin 2
Calculated molecular weight 199aa,22 kDa
Observed molecular weight 22 kDa
GenBank accession number BC002616
Gene symbol TAGLN2
Gene ID (NCBI) 8407
Conjugate Unconjugated
Form Liquid
Purification Method Caprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The transgelin family is a group of proteins that belong to 22kd actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. Our test result showed that this antibody Is specific to TAGLN2. It does not bind TAGLN1 and TAGLN3.

Protocols

Product Specific Protocols
WB protocol for Transgelin 2 antibody 60044-1-IgDownload protocol
IHC protocol for Transgelin 2 antibody 60044-1-IgDownload protocol
IP protocol for Transgelin 2 antibody 60044-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Med Rep

Downregulation of transgelin 2 promotes breast cancer metastasis by activating the reactive oxygen species/nuclear factor‑κB signaling pathway.

Authors - Liu Yang
  • KD Validated