TDP-43 Polyclonal antibody

TDP-43 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 18280-1-AP

Synonyms

ALS10, TAR DNA binding protein, TAR DNA binding protein 43



Tested Applications

Positive WB detected inHeLa cells, K-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inmouse brain tissue, rat brain tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB
Immunohistochemistry (IHC)IHC : 1:200-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

18280-1-AP targets TDP-43 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen fusion protein
Full Name TAR DNA binding protein
Calculated molecular weight 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC001487
Gene symbol TARDBP
Gene ID (NCBI) 23435
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). This antibody recognizes the cleavage product of 20-30 kDa in addition to the native and phosphorylated forms of TDP-43. Immunohistochemical analyses of TDP-43 using this antibody detect both normal diffuse nuclear staining and insoluble inclusions in pathologic tissues.

Protocols

Product Specific Protocols
WB protocol for TDP-43 antibody 18280-1-APDownload protocol
IHC protocol for TDP-43 antibody 18280-1-APDownload protocol
IF protocol for TDP-43 antibody 18280-1-APDownload protocol
IP protocol for TDP-43 antibody 18280-1-APDownload protocol
FC protocol for TDP-43 antibody 18280-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Dis Model Mech

Viral delivery of C9ORF72 hexanucleotide repeat expansions in mice lead to repeat length dependent neuropathology and behavioral deficits.

Authors - Saul Herranz-Martin

Nat Neurosci

TDP-43 extracted from frontotemporal lobar degeneration subject brains displays distinct aggregate assemblies and neurotoxic effects reflecting disease progression rates.

Authors - Florent Laferrière