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PE Anti-Mouse TER-119 (TER-119)

TER-119 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

TER-119

Cat no : PE-65149

Synonyms

Ly76, lymphocyte antigen 76, TER 119, Ter119, TER-119

Validation Data Gallery

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  • Flow cytometry (FC) experiment of mouse bone marrow cells using PE Anti-Mouse TER-119 (TER-119) (PE-65149)

    FC experiment of mouse bone marrow cells using PE-65149

    1X10^6 C57BL/6 mouse bone marrow cells were surface stained with 0.50 ug PE Anti-Mouse TER-119 (PE-65149, Clone: TER-119) (red) or 0.50 ug isotype control antibody (blue). Cells were not fixed.

Tested Applications

Positive FC detected inmouse bone marrow cells

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-65149 targets TER-119 in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Immunogen Cells
Full Name lymphocyte antigen 76
Gene Symbol Ly76
Gene ID (NCBI) 104231
RRIDAB_2883928
Conjugate PE Fluorescent Dye
Excitation/Emission Maxima Wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

TER-119 is a mouse erythroid lineage-specific monoclonal antibody that reacts with erythroid cells at differentiation stages from early proerythroblast to mature erythrocyte, but not with cells showing typical erythroid blast-forming unit (BFU-E) and erythroid colony-forming unit (CFU-E) activities (PMID: 1975515; 10848813). TER-119 recognizes a 52-kDa molecule on erythrocyte membranes (PMID: 10848813). TER-119 antigen is a molecule associated with cell-surface glycophorin A but not with glycophorin A itself (PMID: 10848813).

Protocols

Product Specific Protocols
FC protocol for PE TER-119 antibody PE-65149Download protocol
Standard Protocols
Click here to view our Standard Protocols