Tested Applications
| Positive FC detected in | mouse bone marrow cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
PE-65149 targets TER-119 in FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Cells Predict reactive species |
| Full Name | lymphocyte antigen 76 |
| Gene Symbol | TER-119 |
| Gene ID (NCBI) | 104231 |
| RRID | AB_2883928 |
| Conjugate | PE Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 496 nm, 565 nm / 578 nm |
| Excitation Laser | Blue Laser (488 nm), Green Laser (532 nm), Yellow-Green Laser (561 nm) |
| Form | Liquid |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.09% sodium azide and 0.1% gelatin, pH 7.2. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
TER-119 is a mouse erythroid lineage-specific monoclonal antibody that reacts with erythroid cells at differentiation stages from early proerythroblast to mature erythrocyte, but not with cells showing typical erythroid blast-forming unit (BFU-E) and erythroid colony-forming unit (CFU-E) activities (PMID: 1975515; 10848813). TER-119 recognizes a 52-kDa molecule on erythrocyte membranes (PMID: 10848813). TER-119 antigen is a molecule associated with cell-surface glycophorin A but not with glycophorin A itself (PMID: 10848813).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PE TER-119 antibody PE-65149 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
PE Anti-Mouse TER-119 (Cat# PE-65149) has 3 total citations. These appear in Nephrol Dial Transplant, Inflammopharmacology, and Int Immunopharmacol. The associated research fields center on anemia pathogenesis and immunology, specifically renal anemia, anemia of inflammation, and autoimmune hemolytic anemia in mice, all utilizing flow cytometry applications.
| Species | Application | Title |
|---|---|---|
Nephrol Dial Transplant Unmasking erythroid suppression: the hidden culprit in renal anemia pathogenesis. | ||
Inflammopharmacology Inhibition of alternative complement system and prolyl hydroxylase ameliorates anaemia of inflammation | ||
Int Immunopharmacol Prolyl hydroxylase inhibitor desidustat attenuates autoimmune hemolytic anemia in mice |
