Anti-Mouse TER-119 (TER-119)

• 

  FC experiment of mouse bone marrow cells using 65149-1-Ig

  1x10^6 mouse bone marrow cells were surface stained with 0.5 ug Anti-Mouse TER-119 (65149-1-Ig, Clone: TER-119) or 0.5 ug Rat IgG2b Isotype Control (LTF-2) (65211-1-Ig, Clone: LTF-2), and FITC anti-rat IgG2b Antibody. Cells were co-stained with 0.5 ug CoraLite® Plus 647 Anti-Mouse CD45 (30-F11) (CL647-65087, Clone: 30-F11). Cells were not fixed.

TER-119 Monoclonal Antibody for FC

Cat No. 65149-1-Ig

Clone No.TER-119

Publications(2)

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse and More (1)

Applications

IF, FC

Synonyms

Ly76, lymphocyte antigen 76, TER 119, Ter119, TER-119

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Tested Applications

• Positive FC detected in: mouse bone marrow cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.5 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

65149-1-Ig targets TER-119 in IF, FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Cited Reactivity: mouse
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Day-14 fetal liver cells from a C57BL/6 mouse
• Full Name: lymphocyte antigen 76
• Gene Symbol: TER-119
• Gene ID (NCBI): 104231
• RRID: AB_2918434
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

TER-119 is a mouse erythroid lineage-specific monoclonal antibody that reacts with erythroid cells at differentiation stages from early proerythroblast to mature erythrocyte, but not with cells showing typical erythroid blast-forming unit (BFU-E) and erythroid colony-forming unit (CFU-E) activities (PMID: 1975515; 10848813). TER-119 recognizes a 52-kDa molecule on erythrocyte membranes (PMID: 10848813). TER-119 antigen is a molecule associated with cell-surface glycophorin A but not with glycophorin A itself (PMID: 10848813).

Publications

Species	Application	Title
		Adv Sci (Weinh); Engineered Extracellular Vesicles Driven by Erythrocytes Ameliorate Bacterial Sepsis by Iron Recycling, Toxin Clearing and Inflammation Regulation; Authors - Yan Li
mouse	IF	Thromb Haemost; Platelet-Derived TGF-β1 Promotes Deep Vein Thrombosis; Authors - Sixuan Zhang