Anti-Mouse TER-119 (TER-119)

TER-119 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b

Reactivity

Mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

TER-119

Cat no : 65149-1-Ig

Synonyms

Ly76, lymphocyte antigen 76, TER 119, Ter119, TER-119

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Validation Data Gallery

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  FC experiment of mouse bone marrow cells using 65149-1-Ig

  1X10^6 mouse bone marrow cells were surface stained with 0.5 ug Anti-Mouse TER-119 (65149-1-Ig, Clone: TER-119) and FITC Anti-Rat IgG2b Antibody at dilution 1:200 (green), or stained with 0.5 ug Rat IgG2b Isotype Control and FITC Anti-Rat IgG2b Antibody at dilution 1:200 (black). Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse bone marrow cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

65149-1-Ig targets TER-119 in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Day-14 fetal liver cells from a C57BL/6 mouse
• Full Name: lymphocyte antigen 76
• Gene symbol: Ly76
• Gene ID (NCBI): 104231
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: Phosphate based buffer with 0.09% sodium azide, pH 7.2.
• Storage Conditions: Store at 2-8°C.

Background Information

TER-119 is a mouse erythroid lineage-specific monoclonal antibody that reacts with erythroid cells at differentiation stages from early proerythroblast to mature erythrocyte, but not with cells showing typical erythroid blast-forming unit (BFU-E) and erythroid colony-forming unit (CFU-E) activities (PMID: 1975515; 10848813). TER-119 recognizes a 52-kDa molecule on erythrocyte membranes (PMID: 10848813). TER-119 antigen is a molecule associated with cell-surface glycophorin A but not with glycophorin A itself (PMID: 10848813).