CoraLite®488 Anti-Mouse TER-119 (TER-119)

TER-119 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

TER-119

Cat no : CL488-65149

Synonyms

Ly76, lymphocyte antigen 76, TER 119, Ter119, TER-119



Tested Applications

Positive FC detected inmouse bone marrow cells
For other applications, we recommend the unconjugated version of this antibody, 65149-1-Ig

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

CL488-65149 targets TER-119 in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2b
Class Monoclonal
Type Antibody
Immunogen Day-14 fetal liver cells from a C57BL/6 mouse
Full Name lymphocyte antigen 76
Gene symbol Ly76
Gene ID (NCBI) 104231
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

TER-119 is a mouse erythroid lineage-specific monoclonal antibody that reacts with erythroid cells at differentiation stages from early proerythroblast to mature erythrocyte, but not with cells showing typical erythroid blast-forming unit (BFU-E) and erythroid colony-forming unit (CFU-E) activities (PMID: 1975515; 10848813). TER-119 recognizes a 52-kDa molecule on erythrocyte membranes (PMID: 10848813). TER-119 antigen is a molecule associated with cell-surface glycophorin A but not with glycophorin A itself (PMID: 10848813).

Protocols

Product Specific Protocols
FC protocol for CL488 TER-119 antibody CL488-65149Download protocol
Standard Protocols
Click here to view our Standard Protocols